Image Capture

Introduction

The general consensus in the imaging field when it comes to capturing quality images is simply  

Rubbish in = rubbish out. 

What that means in practice is in order to obtain the best quality images, a number of key steps need to be kept at the front of researcher's minds. These include dye selection and sample prep, choosing the right microscopy method, performing the correct pre-capture alignments and using the right settings for acquisition. 

Important Reading 

• Tutorial- guidance for quantitative confocal microscopy

• The 39 Steps-A Cautionary Tale 

• Fluorescence intensity is not easily quantifiable 

• Colocalisation - What is it and how do I measure it? 

• Magnification, Resolution and Sampling Rates 

• Objective Selection 

• Brightfield Techniques 

• Confocal Theory 

• Confocal imaging speed

• Tiling

• Singe Plane Illumination Techniques (Light-Sheet) 

• Super-Resolution Microscopy 

• FLIM: Fluorescence lifetime imaging microscopy

• Shading Corrections 

• Nyquist Conditions 

• Setting Up Kohler 

• Finding Focus 

• Cleaning Objectives 

• Deconvolution 

Other important information can be found in the following presentation: 

• Introduction to Image Processing