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Institute for Molecular Bioscience Institute for Molecular Bioscience
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Training Manuals

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  4. Microscopy

Analysis Computers

Mac 1-3 Computers running Mac OSX and Windows 7

Hold Down “Option” Key at computer Startup to choose Operating System Analysis

PC’s PC Software: Imaris, AutoQuant, Image Pro Plus, Nikon NIS, ImageJ, Office, NRecon, Dataviewer, Drishti, Zeiss Zen 2009.

MAC Software: Imaris, ImageJ, Office, Drishti.

Mac Pro, 2 x Intel Xeon 4 Core 2.93Ghz CPU, 24Gb RAM, nVidia GTX285 1000Mb GPU, 2 x 320Gb Velociraptor HDD, 2 x 1Tb HDD, 30" LCD monitor.

 

Virtual Computing Machines (VM's) - connect via Remote Desktop via Mac 1-3 or via your desktop/laptop computer.

ImageJ (FIJI), Imaris, Arivis, Microvolution, Zen, LAS-X.

Huygens HyVolution. For more information: Huygens Imaging Academy
 

Connecting to the Analysis VM Guide (PDF)

Confocal Training Manuals

Clicking on the links below will either open or download a .pdf file depending on your browser settings.

  • Confocal 1 (Leica STED) Training Manual

  • Confocal 2 (Zeiss 880 Invert with Fast Airyscan) Training Manual

  • Confocal 3 (Zeiss 710 Upright) Training Manual

  • Confocal 4 (Zeiss 710 Invert) Training Manual

    • AiryScan Training Manual

    • Mai Tai Multi-Photon Laser Training Manual

  • Confocal 5 (Zeiss 710 Invert) Training Manual

  • Zeiss Axiovert Spinning Disc Confocal Training Manual

    • Spinning Disc Camera Alignment Manual

  • Andor Dragonfly Spinning Disc with TIRF

Deconvolution Manuals

Clicking on the links below will either open or download a .pdf file depending on your browser settings.

  • Nikon Deconvolution Training Manual
  • Personal Deltavision Training Manual

Routine Widefield Manuals

Clicking on the links below will either open or download a .pdf file depending on your browser settings.

  • BF/DF Training Manual
  • Basic widefield (Fluoro1, 3-5) Training Manual
  • Fluoro 2 (Zeiss AxioImager with Apotome) Training Manual

Specialty Manuals

Clicking on the links below will either open or download a .pdf file depending on your browser settings.

  • FLIM/FRET Training Manual (to follow)
  • OPT (Optical Projection Tomography) Training Manual

Stereo Manuals

Clicking on the links below will either open or download a .pdf file depending on your browser settings.

  • Stereo 1 Training Manual
  • Stereo 2 Training Manual

IMB Microscopy Resources Online

General Information

Sample Prep

Image Capture

Image Analysis

Visualisation

Structured Training

 

 

  • Confocal Microscopes
  • Hardware and software
  • Publications
  • Resources
  • Image Analysis
  • Lightsheet and Specialty Microscopes
  • Questionnaires
  • Spinning Disc Confocal
  • Stereo Microscopes
  • Widefield Microscopes
  • Widefield microscopes with incubation
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