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Institute for Molecular Bioscience
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How to focus safely

  1. Home
  2. Research
  3. > Research Facilities
  4. Microscopy
  5. Training Manuals
  6. Microscopy Online Resources
  7. Image Capture

Microscope objectives are very expensive and can be damaged very easily, so knowing how to find focus properly is very important.

The video below is a great example on one technique of doing this.

 

How to focus safely

  • Brightfield Techniques
  • Confocal Techniques
  • Deconvolution
  • FLIM: Fluorescence lifetime imaging microscopy
  • How to focus safely
  • How to set up Kohler (Brightfield)
  • Nyquist Conditions
  • Objective Selection
  • Shading Corrections
  • Single Plane Illumination Techniques (Light-sheet Microscopy)
  • Super-Resolution Microscopy
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