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The University of Queensland
Institute for Molecular Bioscience Institute for Molecular Bioscience
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Microscopy Facility

Our standard research services

  • Training users for independent use of the microscopes

  • Experimental design for microscopy experiments

  • Sample preparation consultation

  • 3D design and printing of sample holders

  • Image acquisition

  • Image processing

Our custom research services

  • Lattice light sheet imaging and image processing

  • Fluorescence lifetime imaging and image processing

  • STED imaging

  • Ongoing image analysis training workshops

Systems and equipment available

  • Confocal Microscopes
    • Leica STED 3X Super Resolution Microscope and Fluorescence Lifetime Imaging Microscopy

    • Zeiss LSM 880 Confocal with Fast Airyscan Detector

    • Zeiss LSM 710 Confocal Upright

    • Zeiss LSM 710 Confocal and multiphoton with Airyscan

    • Zeiss LSM 710 Confocal with GaAsP detectors

    • Andor Dragonfly Spinning Disc Confocal with TIRF

    • Zeiss Spinning Disc Confocal

  • Deconvolution microscopes

    • Lattice Lightsheet Microscope

    • Deltavision
    • Nikon Deconvolution

  • Widefield microscopes

    • Brightfield/Darkfield
    • Fluoro 5 Olympus BX51

    • Fluoro 1 Olyupus BX51 Upright

    • Fluoro 2 Zeiss Axioimages Upright

    • Fluoro 3 Olympus IX80 Inverted

    • Fluoro 4 Nikon Ti-U Inverted

  • Stereo microscopes

    • Stereo 1 Olympus SZX12

    • Stereo 2 Nikon SMZ18

    • Stereo 3 Nikon SMZ1500

  • Specialty microscopes

    • Optical Projections Tomography

    • FLIM/FRET

  • Image analysis

    • Virtual Machine

      • 4 machines that each have up to 1.5TB of RAM and 3 GPUs for rapid image analysis

    • Analysis Programs

      • FIJI

      • Imaris

      • Microvolution

      • Huygens professional

      • Arivis

      • Amira

      • FLIMfit

      • LASX

      • Zen Blue

      • Zen Black

      • NIS Elements Viewer

      • TeraStitcher

      • XuvStitch

      • ImarisStitcher

  • Resources

    • Connectivity for IMB users
    • General Information
    • Sample Prep
    • Image Capture
    • Image Analysis
    • Visualisation
    • Structured Training

 

More information

Staff expertise

  • James Springfield​

    • Microscope development, hardware optimization
    • Brightfield and mullti-channel fluorescence microscopy
    • Confocal laser scanning microscopy
    • Rapid, live-cell 3D imaging using spinning disk confocal
    • 2-photon confocal imaging for tissue imaging and micro-ablation experiments
    • Rapid-acquisition live imaging for intracellular processes and live embryo imaging
    • High-throughput imaging and analysis of fluorescence in multi-well plates and dishes

 

  • Nicholas Condon​

    • Lattice Light Sheet microscopy and image processing
    • Brightfield and mullti-channel fluorescence microscopy
    • Confocal laser scanning microscopy
    • Rapid, live-cell 3D imaging using spinning disk confocal
    • 2-photon confocal imaging for tissue imaging and micro-ablation experiments
    • Rapid-acquisition live imaging for intracellular processes and live embryo imaging
    • Image processing and analysis including automation in ImageJ/FIJI

 

  • Deborah Barkauskas

    • Fluorescence lifetime imaging FLIM and analysis
    • Brightfield and mullti-channel fluorescence microscopy
    • Confocal laser scanning microscopy
    • Rapid, live-cell 3D imaging using spinning disk confocal
    • 2-photon confocal imaging for intravital tissue imaging and dynamic analysis
    • Image processing and analysis including automation in ImageJ/FIJI
    • Image processing and analysis using Python and Matlab

Research Groups

  • Alpha Yap

  • Jenny Stow

  • Paul Alewood

  • Chandima Ekanayake

  • Brett Collins

  • Mark Cooper

  • David Craik

  • David Fairlie

  • James Fraser

  • Frederic Gachon

  • Emma Gordon

  • Ben Hankamer

  • Nick Hamilton

  • Ian Henderson

  • Glenn King

  • Anne Lagendijk

  • Richard Lewis

  • Nina Mitter

  • Quan Nguyen

  • Nathan Palpant

  • Rob Parton

  • Kate Schroder

  • Matt Sweet

  • Brandon Wainwright

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