Microscopy Facility
Systems and equipment available
- Confocal Microscopes
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Lattice Lightsheet Microscope
- Deltavision
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Nikon Deconvolution
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- Brightfield/Darkfield
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Fluoro 5 Olympus BX51
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Fluoro 1 Olyupus BX51 Upright
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Fluoro 2 Zeiss Axioimages Upright
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Fluoro 3 Olympus IX80 Inverted
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Fluoro 4 Nikon Ti-U Inverted
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Stereo 1 Olympus SZX12
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Stereo 2 Nikon SMZ18
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Stereo 3 Nikon SMZ1500
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Optical Projections Tomography
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FLIM/FRET
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Image analysis
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Virtual Machine
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4 machines that each have up to 1.5TB of RAM and 3 GPUs for rapid image analysis
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Analysis Programs
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FIJI
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Imaris
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Microvolution
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Huygens professional
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Arivis
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Amira
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FLIMfit
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LASX
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Zen Blue
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Zen Black
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NIS Elements Viewer
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TeraStitcher
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XuvStitch
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ImarisStitcher
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- Connectivity for IMB users
- General Information
- Sample Prep
- Image Capture
- Image Analysis
- Visualisation
- Structured Training
Staff expertise
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James Springfield
- Microscope development, hardware optimization
- Brightfield and mullti-channel fluorescence microscopy
- Confocal laser scanning microscopy
- Rapid, live-cell 3D imaging using spinning disk confocal
- 2-photon confocal imaging for tissue imaging and micro-ablation experiments
- Rapid-acquisition live imaging for intracellular processes and live embryo imaging
- High-throughput imaging and analysis of fluorescence in multi-well plates and dishes
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Nicholas Condon
- Lattice Light Sheet microscopy and image processing
- Brightfield and mullti-channel fluorescence microscopy
- Confocal laser scanning microscopy
- Rapid, live-cell 3D imaging using spinning disk confocal
- 2-photon confocal imaging for tissue imaging and micro-ablation experiments
- Rapid-acquisition live imaging for intracellular processes and live embryo imaging
- Image processing and analysis including automation in ImageJ/FIJI
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Deborah Barkauskas
- Fluorescence lifetime imaging FLIM and analysis
- Brightfield and mullti-channel fluorescence microscopy
- Confocal laser scanning microscopy
- Rapid, live-cell 3D imaging using spinning disk confocal
- 2-photon confocal imaging for intravital tissue imaging and dynamic analysis
- Image processing and analysis including automation in ImageJ/FIJI
- Image processing and analysis using Python and Matlab
Research Groups
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Alpha Yap
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Jenny Stow
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Paul Alewood
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Chandima Ekanayake
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Brett Collins
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Mark Cooper
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David Craik
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David Fairlie
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James Fraser
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Frederic Gachon
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Emma Gordon
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Ben Hankamer
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Nick Hamilton
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Ian Henderson
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Glenn King
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Anne Lagendijk
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Richard Lewis
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Nina Mitter
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Quan Nguyen
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Nathan Palpant
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Rob Parton
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Kate Schroder
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Matt Sweet
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Brandon Wainwright