Confocal 1

Inverted Leica STED 3X Confocal with FLIM

Leica STED Confocal training manual            Location: Rm 6.029a           Bookings           Contact

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Current Status:  Fully Operational

Room 6.029a - Leica DMi8 Inverted Microscope Stand with SP8 Galvo and Resonant Confocal Scanners, White Light Laser (WLL), STED 3X super-resolution, and FLIM/FRET

Inverted point-scanning laser confocal microscope with spectral, super resolution and FLIM/FRET detection. Tuneable 470nm - 670nm WLL, 2 x PMT and 2 x HyD detectors. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features

  • Fully motorised X-Y-Z stage
  • High-sensitivity HyD detectors 
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging
  • Spectral imaging
  • Super-Resolution Imaging with 592nm, 660nm and 775nm STED lasers
  • Fast Resonant Imaging
  • FLIM/FRET Imaging with WLL and 440nm pulsed lasers

 

Transmitted Light:  Leica LED Lamp

Reflected Light:  Leica EL6000 120W White Light Lamp

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Condensor

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.55 28 - K3 K6 K7 - -

 

Objectives

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 HC Plan Apochromat 10x 0.4 Dry 2.2 DIC    
2 HC Plan Apochromat 20x 0.75 Multi 0.67 DIC    
3 HC Plan Apochromat 40x 1.1 Water 0.65 DIC    
                 
5

HC Plan Apochromat(*)

93x 1.30 Glyc 0.3 DIC    
6 HC Plan Apochromat 100x 1.40 Oil 0.13 DIC    

Note: The 93x objective also comes with a motorised correction collar.

 

Fluorescent Filter Sets

Position Name Excitation Dichroic Emission Suitable Dyes
1

DAPI

350/50

 

460/50

DAPI, Hoechst

2

GFP

470/40

 

525/50

EGFP, A488

3

YFP

500/20

 

535/30

YFP

4 Texas Red 560/40   630/75 Texas Red, mCherry

 

Lasers

Laser

Wavelength

Laser Power

BFP Power*

Suitable Dyes

405

405nm

50mW

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DAPI / Hoechst

440 pulsed

440nm

-

-

CFP FLIM/FRET

442

442nm 40mW - CFP
WLL

470nm-670nm

1.5mW

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Visible Dyes

92 STED

592nm

1.5W

-

Star 488, Oregeon Green

660 STED

660nm

1.5W

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Star 470sx, A546

775 STED

775nm

1.5W

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SiR

*BFP = Back Focal Plane of Objective

 

Computer

HP Z640 PC - 3GHz Xeon Processor - 64GB RAM - 24GB nVidia Quadro M6000 GPU

3TB Raid Array

2 x 30" LCD Monitor

 

Software 

Leica LASX Software

Co-localisation, 3D visualisation, LAS Navigator, FRAP, FRET, FALCON FLIM and LIGHTNING Deconvolution.

 

Accessories

Incubation chamber with CO2 and Temperature control

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au


Mr Mark Scott
Senior Microscopist​
  +61 7 334 62042
  mark.scott@uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia

Bookings

UQ staff

External