Dragonfly Spinning Disc

Confocal with TIRF

Location: Rm 6.025b           Bookings           Contact

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Room 6.025b - Nikon Inverted Microscope Stand with Andor Dragonfly Spinning Disc Scanhead

Inverted spinning disc laser confocal microscope, suited for fast live imaging, 3-dimensional multi-position and large scale tiling.  Also has TIRF functionality and incubation.

Features Include:

  • Fully motorised X-Y-Z stage
  • Piezo Z-drive for rapid Z-stacks
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging
  • TIRF imaging
  • Routine time-lapse and ultra-fast burst mode imaging

Transmitted Light:  CoolLED White Light LED

Reflected Light:  Nikon Intensilight 100W White Light Lamp

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Condensor

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD - - - - - - - -

 

Objectives

Position Objective Mag N.A. Imm W.D. (mm) Type R.I. XY Res (nm) Arbitrary Brightness
1 Plan Apo λ 4x 0.2 Dry 20 - 1.00 1488 1
2 Plan Apo λ 10x 0.45 Dry 4.0 DIC II 1.00 662 4
3 Plan Apo VC 20x 0.75 Dry 1.0 DIC II 1.00 397 8
4 Plan Apo λ 40x 0.95 Dry 0.16-0.25 DIC II 1.00 313 5
5 Apo λ LWD 40x 1.15 Water 0.59-0.61 DIC II 1.33 259 11
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Plan Apo λ

60x

1.4

Oil

0.13

Ph3 DM

1.515

213 11

*100x TIRF Oil lens is also available (1.49 N.A.)

 

Laser Lines Available

405, 445, 488, 514, 561 and 637nm

 

Eyepiece Fluorescent Filter Sets

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI 436/25 455 480/40 ECFP, Cerulean
2 GFP 470/40 495 525/50 EGFP, Alexa Fluor 488
3 TRITC 500/25 515 535/30 EYFP, Citrine
4 - - - - -
5 - - - - -
6

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Confocal Fluorescence Dichroic Sets

First Dichroic Set

Second Dichroic Set Third Dichroic Set

405-13

405-13 405-13
488-13 445-13 488-13
561-6 514-14 561-6
640-16 640-16 685-13

 

Confocal Emission Filter Wheel Options

Position Camera 1 Camera 2
1) 445-514 TR-442-514-561
2) 700-75 600-50
3) 620-60 525-50
4) 600-50 450-50
5) 525-50 TR-POL-DIC
6) 450-50 Blocked
7) TR-POL-DIC Blocked
8) Blocked Blocked

 

Computer

Fujitsu PC - 3GHz Xeon Processor - 92GB RAM - 8GB nVidia Quadro GPU

4TB Raid Array

30" LCD Monitor

 

Software

Fusion and Imaris

 

Accessories

Incubation chamber with CO2 and Temperature control

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia

Bookings

UQ staff

External