Dragonfly Spinning Disc

Confocal with TIRF

Location: Rm 6.025b           Bookings           Contact

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Room 6.025b - Nikon Ti2 Inverted Microscope Stand with Andor Dragonfly Spinning Disc Scanhead

Inverted spinning disc laser confocal microscope, suited for fast live imaging, 3-dimensional multi-position and large scale tiling.  Also has TIRF functionality and incubation.

Features Include:

  • Fully motorised X-Y-Z stage
  • 500 um Piezo Z-drive for rapid Z-stacks
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging
  • TIRF imaging
  • Routine time-lapse and ultra-fast burst mode imaging
  • Laser Widefield mode
  • Nikon Perfect Focus 4

Transmitted Light:  CoolLED White Light LED

Reflected Light:  Nikon Intensilight 100W White Light Lamp

Installed in March of 2018 is the Andor Dragonfly incorporates a Spinning Disc Confocal allowing researchers to image 3D volumes at greater speed and over larger areas than ever before. 

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Condensor

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD - - ND - - DIC I DIC II C

 

Objectives

Position Objective Mag N.A. Imm W.D. (mm) Type R.I. XY Res (nm) Arbitrary Brightness
1 Plan Apo 4x 0.2 Dry 20 - 1.00 1488 1
2 Plan Apo λ 10x 0.45 Dry 4.0 DIC I 1.00 662 4
3 Plan Apo VC 20x 0.75 Dry 1.0 DIC II 1.00 397 8
4 Plan Apo λ 40x 0.95 Dry 0.16-0.25 DIC II 1.00 313 5
5 Apo λ LS 40x 1.15 Water 0.59-0.61 DIC II 1.33 259 11
6

Plan Apo λ

60x

1.4

Oil

0.13

Ph3 DM

1.515

213 11

*25x Silicone Oil objective is also available (1.05 N.A.)

*100x TIRF Oil objective is also available (1.49 N.A.)

 

Laser Lines Available

405, 445, 488, 514, 561 and 637nm

 

Eyepiece Fluorescent Filter Sets

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI 375/28 415 460/60 ECFP, Cerulean
2 FITC 480/30 505 535/46 EGFP, Alexa Fluor 488
3 TRITC 540/26 565 605/56 EYFP, Citrine
4 - - - - -
5 - - - - -
6

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- - - -

 

Confocal Fluorescence Dichroic Sets

First Dichroic Set

Second Dichroic Set Third Dichroic Set

405-13

405-13 405-13
488-13 445-13 488-13
561-6 514-14 561-6
640-16 640-16 685-13

 

Simulataneous Acqisition Image Splitter

Image Spliiter
100% pass
500 nm Long Pass
565 nm Long Pass
640 nm Long Pass

 

Confocal Emission Filter Wheel Options

Position Camera 1 Camera 2
1) 620-60 TR-442-514-561
2) 600-50 600-50
3) 542-27 525-50
4) 525-50 483-32
5) 480-70 450-50
6) 450-50 TR-POLR-DIC-FWL
7) TR-POL-DIC-FWL Blocked
8) 700-75 Blocked

 

Computer

Dell Precision 7910 PC - 3.5GHz Xeon Processor - 96GB RAM - 8GB nVidia Quadro M4000 GPU

4TB Raid Array

30" LCD Monitor

 

Software

Fusion and Imaris

 

Accessories

Incubation chamber with CO2 and Temperature control

Single Microscope Slide + 2x35 mm dish insert

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia