Spinning Disc Confocal
Inverted Cell Observer SD
.
Room 6.018 - Zeiss Axiovert 200 Inverted Microscope Stand with CSU-X1 scanhead
Inverted spinning disc laser confocal microscope Suited for live imaging.
Features Include:
- Fully motorised X-Y-Z stage
- Piezo Z-drive for rapid Z-stacks
- CO2 and temperature incubation for live cells and tissue
- Tiled imaging
- Multi-position imaging
- Dual Evolve emCCD cameras
Transmitted Light: Zeiss 100W Halogen Lamp
Reflected Light: Colibri 7 LED light source
.
Condensor
| Name | N.A. | W.D. mm | Position 1 | Position 2 | Position 3 | Position 4 | Position 5 | Position 6 |
| Universal LWD | 0.45 | 54 | Open | PhL | Ph1 | Ph2 | DIC I | DIC II |
Objectives
| Position | Objective | Magnification | N.A. | Immersion | W.D. (mm) | Type | Type | Thread |
| 1 | Plan Apochromat | 40x | 0.95 | Dry | - | DIC III | - | M27 |
| 2 | C Apo | 40x | 1.2 | Water | - | DIC III | M27 | |
| 3 | LCI PlanN | 63x | 1.3 | Water | - | DIC III | M27 | |
| 4 | Plan Apochromat | 10x | 0.45 | Dry | - | DIC II | M27 | |
| 5 | Plan Apochromat | 20x | 0.8 | Dry | - | DIC II | M27 | |
| 6 |
- |
- |
- |
- |
- |
- |
- |
Eyepiece Fluorescent Filter Sets
| Position | Name | Excitation | Dichroic | Emission | Suitable Dyes |
| 1 | CFP (47HE) | 436/25 | 455 | 480/40 | ECFP, Cerulean |
| 2 | GFP (38HE) | 470/40 | 495 | 525/50 | EGFP, Alexa Fluor 488 |
| 3 | YFP (46HE) | 500/25 | 515 | 535/30 | EYFP, Citrine |
| 4 | RFP (63HE) | 572/25 | 590 | 629/62 | mCherry, Cy3 |
| 5 | - | - | - | - | - |
| 6 |
- |
- | - | - | - |
Confocal Fluorescence Filter Sets
|
First Dichroic |
Second Dichroic | Camera1 | Camera2 |
|
405/488/561 |
Empty | 485/30 | 562/45 |
| 457/514/647 | 510LP | 535/30 | 630/98 |
| 405/488/561/647 | 560LP | - | - |
Computer
Fujitsu PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU
3TB Raid Array
30" LCD Monitor
Software
Zeiss Zen 2012 Blue
Accessories
Incubation chamber with CO2 and Temperature control
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
CZI Imaging Scientist
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Mahdie Mollazade
Microscopy Officer
+61 7 334 62042
m.mollazade@uq.edu.au
Mailing address
ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia