Confocal 4

Inverted LSM 710 Meta NLO AiryScan

Confocal 4 training manual             Mai Tai Multiphoton Laser Training Manual            AiryScan Training Manual           Location: Rm 6.034a           Bookings           Contact

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Room 6.034 - Zeiss Axiovert 200 Inverted Microscope Stand with LSM 710 Meta NLO Confocal Scanner

Inverted point-scanning laser confocal microscope with spectral detection and Airyscan super resolution detector. 2-photon imaging with a fully tunable Mai Tai eHP DeepSee 760-1040nm laser with dedicated NDD detectors for high sensitivity. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features Include:

  • Fully motorised X-Y-Z stage
  • Piezo Z-drive for rapid Z-stacks
  • High-sensitivity BiG detectors (GFP/mCherry as wel as a dedicated SHG filter) for 2-photon imaging
  • AiryScan detector for super resolution imaging to 130nm (XY)
  • GaAsP Non-Descanned detectors
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging
  • Spectral imaging
  • Definite focus 1

Transmitted Light:  Zeiss 100W Halogen Lamp

Reflected Light:  Exfo Xcite 120W White Light Lamp

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Condensor

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.55 26 H Ph1 Ph2 Ph3 DIC II DIC III

Objectives

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 10x 0.45 Dry 2.0 DIC II   M27
2 Plan Apochromat 20x 0.8 Dry 0.55 DIC II   M27
3 Plan Apochromat 40x 1.30 Oil 0.21 DIC II   M27
4 Plan Apochromat 63x 1.4 Oil 0.19 DIC II   M27
5 A-Plan Apochromat 100x 1.46 Oil 0.11 DIC II   M27
6 C-Plan Apochromat (Airy) 63x 1.4 Oil 0.14 DIC II   M27

 

Fluorescent Filter Sets

Position Name Excitation Dichroic Emission Suitable Dyes
1

Zeiss #63 HE

572/25

FT 590

629/62

mCherry, Texas Red

2

Zeiss #38 HE

470/40

FT 495

525/50

EGFP, Alexa Fluor 488

3

Zeiss #49 HE

436/25

FT 395

445/50

DAPI, Hoechst

4 - - - - -
5 - - - - -

 

Laser

Laser

Wavelength

Laser Power

BFP Power*

Suitable Dyes

405

405nm

20mW

4.5mW

DAPI, Hoechst

Argon Ion

458nm

1mW

0.4mW

CFP

Argon Ion

488nm

20mW

8mW

GFP, A488

Argon Ion

514nm

3mW

1mW

YFP

561

561nm

10mW

5mW

mCherry, Cy3

633

633nm

5mW

1mW

Cy5, A647

Mai Tai

780 - 1040 nm

2000mW

10mW - 50mW

2 Photon Dyes

*BFP = Back Focal Plane of Objective

 

Computer

Fujitsu PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU

3TB Raid Array

30" LCD Monitor

 

Software

Zeiss Zen 2012 Black

 

Accessories

Incubation chamber with CO2 and Temperature control

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Ms Tevin Chau
Microscopy Officer
  +61 7 334 62042
  c.chau@uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia