Confocal 4

Inverted LSM 710 Meta NLO AiryScan

Confocal 4 training manual             Mai Tai Multiphoton Laser Training Manual            AiryScan Training Manual           Location: Rm 6.034a           Bookings           Contact

.

 

 

 

 

 

Room 6.034 - Zeiss Axiovert 200 Inverted Microscope Stand with LSM 710 Meta NLO Confocal Scanner

Inverted point-scanning laser confocal microscope with spectral detection and Airyscan super resolution detector. 2-photon imaging with a fully tunable Mai Tai eHP DeepSee 760-1040nm laser with dedicated NDD detectors for high sensitivity. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features Include:

  • Fully motorised X-Y-Z stage
  • Piezo Z-drive for rapid Z-stacks
  • High-sensitivity BiG detectors (GFP/mCherry as wel as a dedicated SHG filter) for 2-photon imaging
  • AiryScan detector for super resolution imaging to 130nm (XY)
  • GaAsP Non-Descanned detectors
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging
  • Spectral imaging
  • Definite focus 1

Transmitted Light:  Zeiss 100W Halogen Lamp

Reflected Light:  Exfo Xcite 120W White Light Lamp

.

 

 

 

 

 

Condensor

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.55 26 H Ph1 Ph2 Ph3 DIC II DIC III

Objectives

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 10x 0.45 Dry 2.0 DIC II   M27
2 Plan Apochromat 20x 0.8 Dry 0.55 DIC II   M27
3 Plan Apochromat 40x 1.30 Oil 0.21 DIC II   M27
4 Plan Apochromat 63x 1.4 Oil 0.19 DIC II   M27
5 A-Plan Apochromat 100x 1.46 Oil 0.11 DIC II   M27
6 C-Plan Apochromat (Airy) 63x 1.4 Oil 0.14 DIC II   M27

 

Fluorescent Filter Sets

Position Name Excitation Dichroic Emission Suitable Dyes
1

Zeiss #63 HE

572/25

FT 590

629/62

mCherry, Texas Red

2

Zeiss #38 HE

470/40

FT 495

525/50

EGFP, Alexa Fluor 488

3

Zeiss #49 HE

436/25

FT 395

445/50

DAPI, Hoechst

4 - - - - -
5 - - - - -

 

Accessories

Incubation chamber with CO2 and Temperature control

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia