Confocal 4

Inverted LSM 710 Meta NLO AiryScan

Confocal 4 training manual             Mai Tai Multiphoton Laser Training Manual            AiryScan Training Manual            Zeiss filters            Location: Rm 6.019a           Bookings           Contact

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Room 6.034 - Zeiss Axiovert 200 Inverted Microscope Stand with LSM 710 Meta Confocal Scanner

Inverted point-scanning laser confocal microscope with spectral detection and Airyscan super resolution detector. 2-photon imaging with a fully tunable Mai Tai eHP DeepSee 760-1040nm laser with dedicated BiG(GaAsP) NDD detectors for high sensitivity. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features Include:

  • Fully motorised X-Y-Z stage
  • Piezo Z-drive for rapid Z-stacks
  • High-sensitivity BiG detectors (GFP/mCherry as wel as a dedicated SHG filter) for 2-photon imaging
  • AiryScan detector for super resolution imaging to 130nm (XY)
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging
  • Spectral imaging

Transmitted Light:  Zeiss 100W Halogen Lamp

Reflected Light:  Exfo Xcite 120W White Light Lamp

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Condensor

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.55 26 - - - - - -

Objectives

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 20x 0.8 Dry - -   M27
2 Plan Apochromat 40x 1.3 Oil - -   M27
3 Plan Apochromat 63x 1.40 Oil - -   M27
4 Plan Apochromat 100x 1.46 Oil - -   M27
5 - - - - - -   M27
6 Plan Apochromat 10x 0.45 Dry - -   M27

 

Fluorescent Filter Sets

Position Name Excitation Dichroic Emission Suitable Dyes
1

Zeiss #63 HE

572/25

FT 590

629/62

mCherry, Texas Red

2

Zeiss #38 HE

470/40

FT 495

525/50

EGFP, Alexa Fluor 488

3

Zeiss #47 HE

436/25

FT 455

480/40

DAPI, Hoechst

4 - - - - -
5 - - - - -

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia

Bookings

UQ staff

External