Confocal 4
Inverted LSM 710 Meta NLO AiryScan
.
Room 6.034 - Zeiss Axiovert 200 Inverted Microscope Stand with LSM 710 Meta NLO Confocal Scanner
Inverted point-scanning laser confocal microscope with spectral detection and Airyscan super resolution detector. 2-photon imaging with a fully tunable Mai Tai eHP DeepSee 760-1040nm laser with dedicated NDD detectors for high sensitivity. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.
Features Include:
- Fully motorised X-Y-Z stage
- Piezo Z-drive for rapid Z-stacks
- High-sensitivity BiG detectors (GFP/mCherry as wel as a dedicated SHG filter) for 2-photon imaging
- AiryScan detector for super resolution imaging to 130nm (XY)
- GaAsP Non-Descanned detectors
- CO2 and temperature incubation for live cells and tissue
- Tiled imaging
- Multi-position imaging
- Spectral imaging
- Definite focus 1
Transmitted Light: Zeiss 100W Halogen Lamp
Reflected Light: Exfo Xcite 120W White Light Lamp
.
Condensor
| Name | N.A. | W.D. mm | Position 1 | Position 2 | Position 3 | Position 4 | Position 5 | Position 6 |
| Universal LWD | 0.55 | 26 | H | Ph1 | Ph2 | Ph3 | DIC II | DIC III |
Objectives
| Position | Objective | Magnification | N.A. | Immersion | W.D. (mm) | Type | Type | Thread |
| 1 | Plan Apochromat | 10x | 0.45 | Dry | 2.0 | DIC II | M27 | |
| 2 | Plan Apochromat | 20x | 0.8 | Dry | 0.55 | DIC II | M27 | |
| 3 | Plan Apochromat | 40x | 1.30 | Oil | 0.21 | DIC II | M27 | |
| 4 | Plan Apochromat | 63x | 1.4 | Oil | 0.19 | DIC II | M27 | |
| 5 | A-Plan Apochromat | 100x | 1.46 | Oil | 0.11 | DIC II | M27 | |
| 6 | C-Plan Apochromat (Airy) | 63x | 1.4 | Oil | 0.14 | DIC II | M27 |
Fluorescent Filter Sets
| Position | Name | Excitation | Dichroic | Emission | Suitable Dyes |
| 1 |
Zeiss #63 HE |
572/25 |
FT 590 |
629/62 |
mCherry, Texas Red |
| 2 |
Zeiss #38 HE |
470/40 |
FT 495 |
525/50 |
EGFP, Alexa Fluor 488 |
| 3 |
Zeiss #49 HE |
436/25 |
FT 395 |
445/50 |
DAPI, Hoechst |
| 4 | - | - | - | - | - |
| 5 | - | - | - | - | - |
Laser
|
Laser |
Wavelength |
Laser Power |
BFP Power* |
Suitable Dyes |
|
405 |
405nm |
20mW |
4.5mW |
DAPI, Hoechst |
|
Argon Ion |
458nm |
1mW |
0.4mW |
CFP |
|
Argon Ion |
488nm |
20mW |
8mW |
GFP, A488 |
|
Argon Ion |
514nm |
3mW |
1mW |
YFP |
|
561 |
561nm |
10mW |
5mW |
mCherry, Cy3 |
|
633 |
633nm |
5mW |
1mW |
Cy5, A647 |
|
Mai Tai |
780 - 1040 nm |
2000mW |
10mW - 50mW |
2 Photon Dyes |
*BFP = Back Focal Plane of Objective
Computer
Fujitsu PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU
3TB Raid Array
30" LCD Monitor
Software
Zeiss Zen 2012 Black
Accessories
Incubation chamber with CO2 and Temperature control
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
CZI Scientist
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Deborah Barkauskas
Senior Microscopist
+61 7 334 62042
d.barkauskas@imb.uq.edu.au
Dr Mahdie Mollazade
Microscopy Officer
+61 7 334 62042
m.mollazade@uq.edu.au
Mailing address
ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia