Confocal 3
Upright LSM 710
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Room 6.019a - Zeiss Axio Examiner Upright Microscope Stand with LSM 710 Meta Confocal Scanner
Upright point-scanning laser confocal microscope with spectral detection Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.
Features Include:
- Fully motorised X-Y-Z stage
- Tiled imaging
- Multi-position imaging
- Spectral imaging
Transmitted Light: Zeiss 100W Halogen Lamp
Reflected Light: Exfo Xcite 120W White Light Lamp
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Condensor
| Name | N.A. | W.D. mm | Position 1 | Position 2 | Position 3 | Position 4 | Position 5 | Position 6 |
| - | - | - | - | - | - | - | - | - |
Objectives
| Position | Objective | Magnification | N.A. | Immersion | W.D. (mm) | Type | Type | Thread |
| 1 | Plan Apochromat | 40x | 1.3 | Oil | 0.21 | DIC | - | M27 |
| 2 | Plan Apochromat | 20x | 0.80 | Dry | 0.55 | - | - | M27 |
| 3 | Plan Apochromat | 10x | 0.45 | Dry | 2.1 | - | - | M27 |
| 4 | Plan Apochromat | 63x | 1.4 | Oil | 0.19 | DIC | - | M27 |
Fluorescent Filter Sets
| Position | Name | Excitation | Dichroic | Emission | Suitable Dyes |
| 1 | Zeiss #49 HE | 365 | 395 | 445/50 | DAPI, Hoechst |
| 2 | Zeiss #38 HE | 470/40 | 495 | 525/50 | eGFP, FITC |
| 3 | Zeiss #63 HE | 572/25 | 590 | 545/25 | mCherry, Texas Red |
| 4 | - | - | - | - | - |
| 5 | - | - | - | - | - |
Lasers
|
Laser |
Wavelength |
Laser Power |
BFP Power* |
Suitable Dyes |
|
405 |
405nm |
- |
- |
DAPI, Hoechst |
|
Argon Ion |
458nm |
- | - |
CFP |
|
Argon Ion |
488nm |
- | - |
GFP, Alexa Fluor488 |
|
Argon Ion |
514nm |
- | - |
YFP |
|
561 |
561nm |
- | - |
mCherry, Cy3 |
|
633 |
633nm |
- | - |
Cy5, A647 |
*BFP = Back Focal Plane of Objective
Computer
Fujitsu PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU
3TB Raid Array
30" LCD Monitor
Software
Zeiss Zen 2012 Black
Accessories
Temperature control for dishes
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
CZI Scientist
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Deborah Barkauskas
Senior Microscopist
+61 7 334 62042
d.barkauskas@imb.uq.edu.au
Dr Mahdie Mollazade
Microscopy Officer
+61 7 334 62042
m.mollazade@uq.edu.au
Mailing address
ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia