Confocal 2

Inverted LSM 880 Fast Airyscan

 Confocal 2 training manual           Location: Rm 6.029           Bookings           Contact

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Room 6.029 - Zeiss Axiovert 200 Inverted Microscope Stand with LSM 880 Confocal Scanner with Fast Airyscan Detector

Inverted point-scanning laser confocal microscope with spectral detection.  Suited for imaging fixed samples on slides, samples with low fluorescence, super-resolution Airyscan detection and high-speed live imaging.

Features

  • Fully motorised X-Y-Z stage
  • Tiled imaging
  • Multi-position imaging
  • Spectral imaging
  • Airyscan super-resolution
  • Fast airyscan high-speed imaging

 

Transmitted Light:  Zeiss 100W Halogen Lamp

Reflected Light:  HBO 100W White Light Lamp

 

Another new addition to our arsenal is the Zeiss LSM 880 Confocal, installed in April 2018, this system comprises an inverted microscope with full incubation control for live cell imaging and also features Zeiss' Fast Airyscan detector.  The Airyscan in super-res mode uses a honeycomb detector array that is capable of achieving not only greatly improved signal to noise, but will also allow users to achieve down to 120nm resolution without the need for any changes in dyes or staining protocols. The Fast Airyscan component will also allow for fast imaging of a single fluorescence channel with speeds up to 150 frames per second.

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Condensor

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
- - - - - - - - -

 

Objectives

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 10x 0.45 Dry - -   M27
2 Plan Apochromat 20x 0.80 Dry - - - M27
3 Plan Apochromat 40x 1.3 Oil - -   M27
4 Plan Apochromat 63x 1.4 Oil - -   M27

Note: the 63x Oil objective is hand-picked to be used with the Airy-scan detector as well as a separate 40x Water objective that is also available.

 

Fluorescent Filter Sets

Position Name Excitation Dichroic Emission Suitable Dyes
1 Zeiss #63 HE 572/25 FT 590 629/62 mCherry, Texas Red
2 Zeiss #38 HE 470/40 FT 495 525/50 EGFP, Alexa Fluor 488
3 Zeiss #47 HE 436/25 FT 455 480/40 DAPI, Hoechst
4 - - - - -
5 - - - - -

 

Lasers

Laser

Wavelength

Laser Power

BFP Power*

Suitable Dyes

405

405nm

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-

DAPI / Hoechst

Argon Ion

458nm

- -

CFP

Argon Ion

488nm

- -

GFP / Alexa Fluor488

Argon Ion

514nm

- -

YFP

561

561nm

- -

mCherry / Cy3

633

633nm

- -

Cy5 / A647

*BFP = Back Focal Plane of Objective

 

Computer

Fujitsu PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU

3TB Raid Array

30" LCD Monitor

 

Software

Zeiss Zen 2012 Black

 

Accessories

Incubation chamber with CO2 and Temperature control

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia