Confocal 2

Inverted LSM 880 Fast Airyscan

Zeiss filters            Location: Rm 6.029           Bookings           Contact

.

 

 

 

 

 

Room 6.029 - Zeiss Axiovert 200 Inverted Microscope Stand with LSM 880 Confocal Scanner with Fast Airyscan Detector

Inverted point-scanning laser confocal microscope with spectral detection.  Suited for imaging fixed samples on slides, samples with low fluorescence, super-resolution Airyscan detection and high-speed live imaging.

Features

  • Fully motorised X-Y-Z stage
  • Tiled imaging
  • Multi-position imaging
  • Spectral imaging
  • Airyscan super-resolution
  • Fast airyscan high-speed imaging

 

Transmitted Light:  Zeiss 100W Halogen Lamp

Reflected Light:  HBO 100W White Light Lamp

 

.

 

 

 

 

 

Condensor

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
- - - - - - - - -

 

Objectives

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 10x 0.45 Dry - -   M27
2 Plan Apochromat 20x 0.80 Dry - - - M27
3 Plan Apochromat 40x 1.3 Oil - -   M27
4 Plan Apochromat 63x 1.4 Oil - -   M27

Note: the 63x Oil objective is hand-picked to be used with the Airy-scan detector as well as a separate 40x Water objective that is also available.

 

Fluorescent Filter Sets

Position Name Excitation Dichroic Emission Suitable Dyes
1 Zeiss #63 HE 572/25 FT 590 629/62 mCherry, Texas Red
2 Zeiss #38 HE 470/40 FT 495 525/50 EGFP, Alexa Fluor 488
3 Zeiss #47 HE 436/25 FT 455 480/40 DAPI, Hoechst
4 - - - - -
5 - - - - -

 

Lasers

Laser

Wavelength

Laser Power

BFP Power*

Suitable Dyes

405

405nm

-

-

DAPI / Hoechst

Argon Ion

458nm

- -

CFP

Argon Ion

488nm

- -

GFP / Alexa Fluor488

Argon Ion

514nm

- -

YFP

561

561nm

- -

mCherry / Cy3

633

633nm

- -

Cy5 / A647

*BFP = Back Focal Plane of Objective

 

Computer

Fujitsu PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU

3TB Raid Array

30" LCD Monitor

 

Software

Zeiss Zen 2012 Black

 

Accessories

Incubation chamber with CO2 and Temperature control

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au


Mr Mark Scott
Senior Microscopist​
  +61 7 334 62042
  mark.scott@uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia

Bookings

UQ staff

External