Confocal 5

Inverted LSM 710 BIG

Confocal 5 training manual            Location: Rm 6.028           Bookings           Contact

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Room 6.028 - Zeiss Axiovert 200 Inverted Microscope Stand with LSM 710 Confocal Scanner with BiG detectors

Inverted point-scanning laser confocal microscope.  Dedicated BiG(GaAsP) detectors for increased sensitivity. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging. 

Features

  • Fully motorised X-Y-Z stage
  • High-sensitivity BiG detectors (GFP/mCherry and CFP/YFP) 
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging
  • Spectral imaging

 

Transmitted Light:  Zeiss 100W Halogen Lamp

Reflected Light:  Exfo Xcite 120W White Light Lamp

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Condensor

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.55 26 - - - - - -

Objectives

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 10x 0.45 Dry 2.1 - - M27
2 Plan Apochromat 20x 0.8 Dry 0.55 - - M27
3 EC Plan-Neofluar 40x 0.75 Dry 0.71 - - M27
4 LD C-Apochromat 40x 1.1 Water 0.62 - - M27
5 LD C-Apochromat 63x 1.15 Water 0.6 - - M27
6 Plan Apochromat 63x 1.4 Oil 0.19 - - M27

 

Fluorescent Filter Sets

Position Name Excitation Dichroic Emission Suitable Dyes
1 Zeiss #49 HE 365 395 445/50 DAPI
2 Zeiss #47 HE 436/25 455 480/40 CFP
3 Zeiss #38 HE 470/40 495 525/50 eGFP, FITC
4 Zeiss #43 HE 545/25 570 605/70 mCherry, Texas Red
5 - - - - -
6 - - - - -

 

Lasers

Laser

Wavelength

Laser Power

BFP Power*

Suitable Dyes

405

405nm

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DAPI, Hoechst

Argon Ion

458nm

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CFP

Argon Ion

488nm

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GFP, A488

Argon Ion

514nm

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YFP

561

561nm

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mCherry, Cy3

594

594nm

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A594

633

633nm

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Cy5, A647

*BFP = Back Focal Plane of Objective

 

Computer

Fujitsu PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU3TB Raid Array

30" LCD Monitor

 

Software 

Zeiss Zen 2012 Black

 

Accessories

Incubation chamber with CO2 and Temperature control

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Ms Tevin Chau
Microscopy Officer
  +61 7 334 62042
  c.chau@uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia