Confocal 5
Inverted LSM 710 BIG
.
Room 6.028 - Zeiss Axiovert 200 Inverted Microscope Stand with LSM 710 Confocal Scanner with BiG detectors
Inverted point-scanning laser confocal microscope. Dedicated BiG(GaAsP) detectors for increased sensitivity. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.
Features
- Fully motorised X-Y-Z stage
- High-sensitivity BiG detectors (GFP/mCherry and CFP/YFP)
- CO2 and temperature incubation for live cells and tissue
- Tiled imaging
- Multi-position imaging
- Spectral imaging
Transmitted Light: Zeiss 100W Halogen Lamp
Reflected Light: Exfo Xcite 120W White Light Lamp
.
Condensor
| Name | N.A. | W.D. mm | Position 1 | Position 2 | Position 3 | Position 4 | Position 5 | Position 6 |
| Universal LWD | 0.55 | 26 | - | - | - | - | - | - |
Objectives
| Position | Objective | Magnification | N.A. | Immersion | W.D. (mm) | Type | Type | Thread |
| 1 | Plan Apochromat | 10x | 0.45 | Dry | 2.1 | - | - | M27 |
| 2 | Plan Apochromat | 20x | 0.8 | Dry | 0.55 | - | - | M27 |
| 3 | EC Plan-Neofluar | 40x | 0.75 | Dry | 0.71 | - | - | M27 |
| 4 | LD C-Apochromat | 40x | 1.1 | Water | 0.62 | - | - | M27 |
| 5 | LD C-Apochromat | 63x | 1.15 | Water | 0.6 | - | - | M27 |
| 6 | Plan Apochromat | 63x | 1.4 | Oil | 0.19 | - | - | M27 |
Fluorescent Filter Sets
| Position | Name | Excitation | Dichroic | Emission | Suitable Dyes |
| 1 | Zeiss #49 HE | 365 | 395 | 445/50 | DAPI |
| 2 | Zeiss #47 HE | 436/25 | 455 | 480/40 | CFP |
| 3 | Zeiss #38 HE | 470/40 | 495 | 525/50 | eGFP, FITC |
| 4 | Zeiss #43 HE | 545/25 | 570 | 605/70 | mCherry, Texas Red |
| 5 | - | - | - | - | - |
| 6 | - | - | - | - | - |
Lasers
|
Laser |
Wavelength |
Laser Power |
BFP Power* |
Suitable Dyes |
|
405 |
405nm |
- |
- |
DAPI, Hoechst |
|
Argon Ion |
458nm |
- |
- |
CFP |
|
Argon Ion |
488nm |
- |
- |
GFP, A488 |
|
Argon Ion |
514nm |
- |
- |
YFP |
|
561 |
561nm |
- |
- |
mCherry, Cy3 |
|
594 |
594nm |
- |
- |
A594 |
|
633 |
633nm |
- |
- |
Cy5, A647 |
*BFP = Back Focal Plane of Objective
Computer
Fujitsu PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU3TB Raid Array
30" LCD Monitor
Software
Zeiss Zen 2012 Black
Accessories
Incubation chamber with CO2 and Temperature control
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
Research Officer, Microscopy
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Deborah Barkauskas
Senior Microscopist
+61 7 334 62042
d.barkauskas@imb.uq.edu.au
Dr Tevin Chau
Microscopy Officer
+61 7 334 62042
c.chau@uq.edu.au
Mailing address
ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia