FPbase.org

Background 

Access Link:   https://www.fpbase.org/ 

FPbase was made by Talley Lambert from Harvard University as a one-stop shop for researchers to plan their imaging experiments. 

The website has a trove of information on fluorescent proteins and dyes including sequences, spectra, evolution, lifetime, quantum yield etc. 

Fluorescent protein Database: 

For example the following page on eGFP: https://www.fpbase.org/protein/egfp/ 

Spectra Viewer 

You can also plan your experiments with respect to the dyes you may choose to use. 

Consider the following set up with mCherry + eGFP.  Depending on your experience level and knowledge of how the microscopes work, you can add in light-sources, filters, and detectors to determine efficiencies as well.