Fluoro 4
Inverted Nikon Ti-U
Nikon Ti-U Inverted Microscope Stand with Nikon DS-Fi1 and Qi1 CCD cameras
Inverted widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.
Features Include:
- Manual X-Y-Z stage
Transmitted Light: Nikon 100W Halogen Lamp
Reflected Light: Nikon 100W Mercury White Lightsource
Condensor:
| Name | N.A. | W.D. mm | Position 1 | Position 2 | Position 3 | Position 4 | Position 5 | Position 6 |
| Universal LWD | 0.45 | 54 | Open | PhL | Ph1 | Ph2 | DIC I | DIC II |
Objectives:
| Position | Objective | Magnification | N.A. | Immersion | W.D. (mm) | Type | Type |
| 1 | Plan Apochromat | 2x | 0.1 | Dry | 8.5 | - | - |
| 2 | Plan Fluor | 4x | 0.13 | Dry | 17.2 | PhL | - |
| 3 | Plan Fluor | 10x | 0.30 | Dry | 16 | Ph1 | - |
| 4 | S Plan Fluor | 20x | 0.45 | Dry | 7.4 | Ph1 | - |
| 4 | S Plan Fluor | 40x | 0.60 | Dry | 3.6-2.8 | Ph2 | - |
| 5 | Plan Fluor | 100x | 1.30 | Oil | 0.16 | Ph3 | - |
| 6 | - | - | - | - | - | - | - |
Fluorescent Filter Sets:
| Position | Name | Excitation | Dichroic | Emission | Suitable Dyes |
| 1 | DAPI - 5060C | 377/50 | 409LP | 447/60 | DAPI, Hoechst |
| 2 | CFP - 2432C | 438/24 | 458LP | 483/32 | ECFP, Cerulean |
| 3 | GFP-3000B | 488/10 | 505LP | 530/60 | EGFP, Alexa Fluor 488 |
| 4 | YFP-2427B | 500/24 | 520LP | 542/27 | EYFP, mVenus |
| 5 | mCherry-40LP-A | 562/40 | 593LP | 593LP | mCherry Alexa Fluor 568 |
| 6 | - | - | - | - | - |
Computer:
Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU
1TB Raid Array
30" LCD Monitor
Software:
NIS Elements-BR, NIS Elements F
Accessories:
Motorised Z-Focus
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
CZI Scientist
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Deborah Barkauskas
Senior Microscopist
+61 7 334 62042
d.barkauskas@imb.uq.edu.au
Dr Mahdie Mollazade
Microscopy Officer
+61 7 334 62042
m.mollazade@uq.edu.au
Mailing address
ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia