Fluoro 4

Inverted Nikon Ti-U

Fluoro 4 Training Manual          Location: Rm 3.022           Bookings

 

 

 

 

 

 

Nikon Ti-U Inverted Microscope Stand with Nikon DS-Fi1 and Qi1 CCD cameras

Inverted widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.

Features Include:

  • Manual X-Y-Z stage

Transmitted Light:  Nikon 100W Halogen Lamp

Reflected Light:  Nikon 100W Mercury White Lightsource

 

 

 

 

 

 

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.45 54 Open PhL Ph1 Ph2 DIC I DIC II

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type
1 Plan Apochromat 2x 0.1 Dry 8.5 - -
2 Plan Fluor 4x 0.13 Dry 17.2 PhL -
3 Plan Fluor 10x 0.30 Dry 16 Ph1 -
4 S Plan Fluor 20x 0.45 Dry 7.4 Ph1 -
4 S Plan Fluor 40x 0.60 Dry 3.6-2.8 Ph2 -
5 Plan Fluor 100x 1.30 Oil 0.16 Ph3 -
6 - - - - - - -

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI - 5060C 377/50 409LP 447/60 DAPI, Hoechst
2 CFP - 2432C 438/24 458LP 483/32 ECFP, Cerulean
3 GFP-3000B 488/10 505LP 530/60 EGFP, Alexa Fluor 488
4 YFP-2427B 500/24 520LP 542/27 EYFP, mVenus
5 mCherry-40LP-A 562/40 593LP 593LP mCherry Alexa Fluor 568
6 - - - - -

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

30" LCD Monitor

Software: 

NIS Elements-BR, NIS Elements F

Accessories:

Motorised Z-Focus

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Ms Tevin Chau
Microscopy Officer
  +61 7 334 62042
  c.chau@uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia