Queensland Facility for Advanced Genome Editing

OUR FACILITY

The Queensland Facility for Advanced Genome Editing (QFAGE) is a core facility at the Institute for Molecular Bioscience (IMB), the University of Queensland.

Together with Transgenic Animal Service of Queensland (TASQ), we provide academic customers expert genetic modification (GM) services in rodent and cell lines using advanced CRISPR genome editing technology. So far, we have successfully generated more than 58 CRISPR mice models and edited genes in 27 different cell types including stem cells (human iPSC) and different cancer cell lines with high efficiency. In addition to our CRISPR services, we also offer lentivirus packaging and production services for UQ internal customers

 

OUR SERVICES

CRISPR editing services

  • Gene knockout
  • Large genomic deletions
  • Generation of small, specific changes (e.g. point mutations, small specific insertions/deletions)
  • Epitope tagging
  • Generation of conditional (“floxed”) alleles
  • Insertion of large DNA fragments or reporters.
  • Safe harbor knock-in (AAVS1, Rosa26)
  • Traditional transgenesis

Standard CRISPR/Cas9 and cell line production service involves three staged modules:

  • Module 1: pre-experiment design, reagent preparation, and cell line QC
  • Module 2: transfection and pool efficiency analysis
  • Module 3: Single colony generation, analysis and handover stage

In general, we provide the following types of services (the cost for the standard service varies according to the type of experiment involved – please contact us for detailed pricing).

Type 1 CRISPR/Cas9 project (simple): Gene knockout or large deletion. This normally involves targeting two sgRNAs either side of the region or exon to be deleted, and no repair template, so the deletion results from non-homologous end joining (NHEJ).

Type 2 CRISPR/Cas9 project (intermediate): Generation of small, specific changes (e.g. point mutations, small specific insertions/deletions up to 60 bp, or epitope tagging). This normally involves using two sgRNAs targeted at the site of interest, and homology-director repair (HDR) via a single-stranded DNA oligonucleotide substrate (~150 bases).

Type 3 CRISPR/Cas9 project (complex): Generation of conditional (“floxed”) alleles or insertion of large DNA fragments. These experiments are much more complicated and involve either several sgRNAs (two for each LoxP insertion site) and repair templates, or a large plasmid DNA substrate (5–10 kb) to either “flox” an exon or “knock-in” a reporter or other large piece of sequence.

Lentivirus packaging and production services (only for UQ internal customers)

QFAGE offers a lentivirus packaging and production service which provides customers with ready-to-use lentivirus to overexpress or knockdown (shRNA) mouse and human genes of interest. QFAGE titers typically have ≥ 108 transducing unit per ml (titration performed with mouse fibroblasts). We can also assist in selection of the optimal vector design and cloning if required.

For more details of our service, please contact qfage@imb.uq.edu.au or submit your EOI form from the links below.

 

OUR AIMS

QFAGE’s service combines both of our experience in GM model production, cell line engineering and genome editing and assures a quality service to maximize your research success.

QFAGE operates as a cost-recovery and pay as you go service. We aim to provide competitive and affordable prices for all researchers. We offer a flexible service to suit your experimental needs, level of experience and budget.

Contacts