The IMB Sequencing Facility (ISF) provides sequencing services to UQ and the research community in the greater Brisbane region.
The sequencing specialist team will tailor the sequencing aspect of your research project to your needs and maintain active communication throughout the course of the project.
We can usually provide fast turnaround times for most projects with a track record of returning high-quality sequencing data within three weeks of sample submission.
Let our team help you with library preparation and sequencing on Illumina short read sequencing platforms and on the PacBio long read sequencing platform Sequel II.
Our standard sequencing services
Our standard library preparation and sequencing services include
- Illumina (short read) whole transcriptome sequencing for gene expression profiling or deeper transcriptome analysis
- 10x Genomics Chromium Single Cell 3' Gene Expression (including CITE-seq/cell hashing)
- PacBio whole genome sequencing (long reads and HiFi reads)
- PacBio IsoSeq (full length transcript analysis)
Our custom sequencing services
In addition, we are happy to discuss how we can help with your custom sequencing requirements. We have experience with library prep and/or sequencing in the following areas:
- Exome and custom capture
- Illumina sequencing panel
- Amplicon sequencing
- Sequencing customer-prepared libraries
- 10x Genomics Chromium VDJ and Single Cell ATAC assays
- Spatial transcriptomics
- ATAC-seq
- CAGE
- Small RNA
- Hi-C
Systems and Equipment available
Software
- We convert your Illumina short read raw sequencing data into fastQ files running the bcl2fastq conversion.
- We will be able to generate fastQ files and count table for your chromium single cell experiment using the cell ranger software.
Staff expertise
Staff has extensive knowledge of Next-generation-sequencing in all areas.
- Single cell
- RNA/DNA
- Short and Long read sequencing
Contacts
Sequencing Facility Manager
Ms Angelika Christ
a.christ@uq.edu.au
+61 7 334 62074
Sequencing Specialist
Dr Tim Bruxner
t.bruxner@uq.edu.au
+61 7 334 62074
Senior Research Assistant
Ms Stacey Andersen
s.andersen2@uq.edu.au
+61 7 334 62074
General enquires
+61 7 334 62074
seq-core@imb.uq.edu.au
Mailing address
IMB Sequencing Facility
Institute for Molecular Bioscience
Level 5N
Building 80
306 Carmody Road
University of Queensland
4072, St Lucia,
Queensland, Australia
Bookings
Please contact the IMB Sequencing Facility seq-core@imb.uq.edu.au