Specialty
FLIM/FRET
Current Status: Fully Operational
Room 2.138 - Olympus IX71 - Inverted Microscope Stand with Intensified CCD camera
Inverted widefield microscope with Mercury and LED lightsources and CCD camera. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.
Features
- Manual X-Y-Z stage
- CO2 and temperature incubation for live cells and tissue
- Tiled imaging
- Multi-position imaging
Transmitted Light: 100W Halogen Lamp
Reflected Light: Olympus Mercury White Lightsource, Lambert LED Lightsource
Objectives
| Position | Objective | Magnification | N.A. | Immersion | W.D. (mm) | Type | Type | Thread |
| 1 | Plan Apochromat | 4x | 0.5 | Dry | 2 | PhL | ADL | M27 |
| 2 | Plan Apochromat | 10x | 0.75 | Dry | 0.5 | Ph1 | M27 | |
| 3 | Plan Apochromat | 20x | 0.75 | Dry | 0.4 | Ph2 | ADL | M27 |
| 4 | Plan Apochromat | 40x | 0.95 | Dry | 0.2 | DIC I | M27 |
Fluorescent Filter Sets
| Position | Name | Excitation | Dichroic | Emission | Suitable Dyes |
| 1 | DAPI - 5060C | 377/50 | 409LP | 447/60 | DAPI, Hoechst |
| 2 | CFP - 2432C | 438/24 | 458LP | 483/32 | CFP, Cerulean |
| 3 | GFP-3000B | 488/10 | 505LP | 530/60 | GFP, A488 |
| 4 | mCherry LP | 560/20 | 580LP | 585LP | mCherry, Cy3 |
| 5 | - | - | - | ||
| 6 | - | - | - |
Computer
Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU
1TB Raid Array
Dual 27" LCD Monitors
Software
Lambert Intruments
Accessories
Incubation chamber with CO2 and Temperature control
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
CZI Imaging Scientist
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Mahdie Mollazade
Microscopy Officer
+61 7 334 62042
m.mollazade@uq.edu.au
Mailing address
ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia