Fluoro 5
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Olympus BX51 Upright Microscope Stand with Nikon DS-Fi3 sCMOS camera
Upright widefield microscope with halogen lightsource and sCMOS camera. Suited for imaging fixed fluorescent samples.
Features Include:
- Manual X-Y-Z stage
- Brightfield, phase-contrast and darkfield
- Fluorescence
- Colour Camera
Transmitted Light: Olympus 100W Halogen Lamp
Reflected Light: Nikon Intensilight 130W Halogen White Lightsource
Condensor:
| Name | N.A. | W.D. mm | Position 1 | Position 2 | Position 3 | Position 4 | Position 5 |
| Universal LWD | 0.45 | 54 | Open | Ph1 | Ph2 | Ph3 | empty |
Objectives:
| Position | Objective | Magnification | N.A. | Immersion | W.D. (mm) | Type | Type | Thread |
| 1 | U Plan FL | 4x | 0.13 | Dry | 17 | PhL | - | M23 |
| 2 | U Plan FL N | 10x | 0.3 | Dry | 10 | Ph1 | - | M23 |
| 3 | LUC Plan FL N | 20x | 0.45 | Dry | 6.6 | Ph1 | - | M23 |
| 4 | U Plan S Apochromat | 40x | 0.95 | Dry | 0.18 | - | - | M23 |
| 5 | U Plan S Apochromat | 60x | 1.35 | Oil | 0.15 | - | - | M23 |
| 6 | U Plan S Apochromat | 100x | 1.4 | Oil | 0.13 | - | - | M23 |
Fluorescent Filter Sets:
| Position | Name | Excitation | Dichroic | Emission | Suitable Dyes |
| 1 | DAPI - U-MNUA2 | 365/10 | 400LP | 440/40 | DAPI, Hoechst |
| 2 | GFP-4050B | 466/40 | 495LP | 525/50 | EGFP, Alexa Fluor 488 |
| 3 | mCherry-40LP-A | 562/40 | 593LP | 593LP |
mCherry, Alexa Fluor 568 |
| 4 | CFP-2432C | 438/24 | 458LP | 483/32 | ECFP, mCerulean |
| 5 | YFP-2427B | 535/30 | 550LP | 570/30 | EYFP, mVenus |
| 6 | Empty | - | - | - | Brightfield |
Computer:
Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU
1TB Raid Array
42" LCD Monitor
Software:
NIS Elements F
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
CZI Imaging Scientist
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Mahdie Mollazade
Microscopy Officer
+61 7 334 62042
m.mollazade@uq.edu.au
Mailing address
Advanced Microscopy Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia