Fluoro 5
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Olympus BX51 Upright Microscope Stand with Photometrics Cool Snap ES2 CCD camera
Upright widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.
Features Include:
- Manual X-Y-Z stage
Transmitted Light: Olympus 100W Halogen Lamp
Reflected Light: XCite 120Q Halogen White Lightsource
Condensor:
| Name | N.A. | W.D. mm | Position 1 | Position 2 | Position 3 | Position 4 | Position 5 |
| Universal LWD | 0.45 | 54 | Open | Ph1 | Ph2 | Ph3 | empty |
Objectives:
| Position | Objective | Magnification | N.A. | Immersion | W.D. (mm) | Type | Type | Thread |
| 1 | U Plan FL | 4x | 0.13 | Dry | 17 | PhL | - | M23 |
| 2 | U Plan FL N | 10x | 0.3 | Dry | 10 | Ph1 | - | M23 |
| 3 | LUC Plan FL N | 20x | 0.45 | Dry | 6.6 | Ph1 | - | M23 |
| 4 | U Plan S Apochromat | 40x | 0.95 | Dry | 0.18 | - | - | M23 |
| 5 | U Plan S Apochromat | 60x | 1.35 | Oil | 0.15 | - | - | M23 |
| 6 | U Plan S Apochromat | 100x | 1.4 | Oil | 0.13 | - | - | M23 |
Fluorescent Filter Sets:
| Position | Name | Excitation | Dichroic | Emission | Suitable Dyes |
| 1 | DAPI - U-MNUA2 | 365/10 | 400LP | 440/40 | DAPI, Hoechst |
| 2 | GFP-4050B | 466/40 | 495LP | 525/50 | EGFP, Alexa Fluor 488 |
| 3 | mCherry-40LP-A | 562/40 | 593LP | 593LP |
mCherry, Alexa Fluor 568 |
| 4 | CFP-2432C | 438/24 | 458LP | 483/32 | ECFP, mCerulean |
| 5 | YFP-2427B | 535/30 | 550LP | 570/30 | EYFP, mVenus |
| 6 | Empty | - | - | - | Brightfield |
Computer:
Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU
1TB Raid Array
42" LCD Monitor
Software:
NIS Elements F
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
CZI Imaging Scientist
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Mahdie Mollazade
Microscopy Officer
+61 7 334 62042
m.mollazade@uq.edu.au
Mailing address
ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia