Fluoro 5

FL5 Training Guide            Location: Rm 6.033           Bookings

 

 

 

 

 

 

Olympus BX51 Upright Microscope Stand with Photometrics Cool Snap ES2 CCD camera 

Upright widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.

Features Include:

  • Manual X-Y-Z stage

Transmitted Light:  Olympus 100W Halogen Lamp

Reflected Light:  XCite 120Q Halogen White Lightsource

 

 

 

 

 

 

 

 

 

 

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5
Universal LWD 0.45 54 Open Ph1 Ph2 Ph3 empty

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 U Plan FL 4x 0.13 Dry 17 PhL - M23
2 U Plan FL N 10x 0.3 Dry 10 Ph1 - M23
3 LUC Plan FL N 20x 0.45 Dry 6.6 Ph1 - M23
4 U Plan S Apochromat 40x 0.95 Dry 0.18 - - M23
5 U Plan S Apochromat 60x 1.35 Oil 0.15 - - M23
6 U Plan S Apochromat 100x 1.4 Oil 0.13 - - M23

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI - U-MNUA2 365/10 400LP 440/40 DAPI, Hoechst
2 GFP-4050B 466/40 495LP 525/50 EGFP, Alexa Fluor 488
3 mCherry-40LP-A 562/40 593LP 593LP

mCherry, Alexa Fluor 568

4 CFP-2432C 438/24 458LP 483/32 ECFP, mCerulean
5 YFP-2427B 535/30 550LP 570/30 EYFP, mVenus
6 Empty - - - Brightfield

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

42" LCD Monitor

Software: 

NIS Elements F

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Ms Tevin Chau
Microscopy Officer
  +61 7 334 62042
  c.chau@uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia