Fluoro 3

Inverted Olympus IX80

FL3 Training Guide            Location: Rm 6.033           Bookings

 

 

 

 

 

 

Olympus IX80 Inverted Microscope Stand with Photometrics Cool Snap HQ2 CCD camera

Inverted widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.

Features Include:

  • Manual X-Y-Z stage

Transmitted Light:  Olympus 100W Halogen Lamp

Reflected Light:  XCite 120Q Halogen White Lightsource

 

 

 

 

 

 

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.45 54 Open PhL Ph1 Ph2 DIC I DIC II

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 4x 0.5 Dry 2 PhL ADL M27
2 Plan Apochromat 10x 0.75 Dry 0.5 Ph1   M27
3 Plan Apochromat 20x 0.75 Dry 0.4 Ph2 ADL M27
4 Plan Apochromat 40x 0.95 Dry 0.2 DIC I   M27
5 Plan Apochromat 63x 1.4 Oil 0.17 DIC II   M27
6 Plan Apochromat 100x 1.4 Oil 0.13 DIC III   M27

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI - 5060C 377/50 409LP 447/60 DAPI, Hoechst
2 CFP - 2432C 438/24 458LP 483/32 CFP, Cerulean
3 GFP-3000B 488/10 505LP 530/60 GFP, A488
4 mCherry LP 560/20 580LP 585LP mCherry, Cy3
5   - - -  
6   - - -  

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

47" TV Monitor

Software: 

NIS Elements F

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia