Fluoro 3
Inverted Olympus IX80
Olympus IX80 Inverted Microscope Stand with Photometrics Cool Snap HQ2 CCD camera
Inverted widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.
Features Include:
- Manual X-Y-Z stage
Transmitted Light: Olympus 100W Halogen Lamp
Reflected Light: XCite 120Q Halogen White Lightsource
Condensor:
| Name | N.A. | W.D. mm | Position 1 | Position 2 | Position 3 | Position 4 | Position 5 | Position 6 |
| Universal LWD | 0.45 | 54 | Open | PhL | Ph1 | Ph2 | DIC I | DIC II |
Objectives:
| Position | Objective | Magnification | N.A. | Immersion | W.D. (mm) | Type | Type | Thread |
| 1 | U Plan Fluorite | 4x | 0.13 | Dry | 17 | M23 | ||
| 2 | U Plan Apochromat | 10x | 0.4 | Dry | 3.1 | M23 | ||
| 3 | U Plan S Apochromat | 20x | 0.75 | Dry | 0.65 | M23 | ||
| 4 | U Plan FLN | 40x | 0.75 | Dry | 0.51 | M23 | ||
| 5 | U Plan S Apochromat | 63x | 1.35 | Oil | 0.17 | M23 | ||
| 6 | U Plan Apochromat | 100x | 1.35 | Oil | 0.10 | DIC III | M23 |
Fluorescent Filter Sets:
| Position | Name | Excitation | Dichroic | Emission | Suitable Dyes |
| 1 | DAPI - 5060C | 377/50 | 409LP | 447/60 | DAPI, Hoechst |
| 2 | CFP - 2432C | 438/24 | 458LP | 483/32 | CFP, Cerulean |
| 3 | GFP-3000B | 488/10 | 505LP | 530/60 | GFP, A488 |
| 4 | YFP-2427B | 500/24 | 520 | 542/27 | YFP |
| 5 | mCherry-40LP | 560/20 | 580LP | 585LP | mCherry, Cy3 |
| 6 | - | - | - | - | - |
Computer:
Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU
1TB Raid Array
47" TV Monitor
Software:
NIS Elements F
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
CZI Imaging Scientist
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Mahdie Mollazade
Microscopy Officer
+61 7 334 62042
m.mollazade@uq.edu.au
Mailing address
ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia