Fluoro 1
Upright Olympus BX51
Olympus BX51 Upright Microscope Stand with Nikon Digital Sight DS-Fi3 CCD camera
Upright widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.
Features Include:
- Manual X-Y-Z stage
- Polarised light
- Differential Interference Contrast (DIC)
- Optovar (1x, 1.25x, 1.6x, 2x)
Transmitted Light: Olympus 100W Halogen Lamp
Reflected Light: XCite 120Q Halogen White Lightsource
Condensor:
| Name | N.A. | W.D. mm | Position 1 | Position 2 | Position 3 | Position 4 | Position 5 | Position 6 |
Position 7 |
Position 8 |
| Universal | 0.9 | - | Open | Open | Open | 10x DIC | 20x DIC | 40x DIC | 60x DIC | 100x DIC |
Objectives:
| Position | Objective | Magnification | N.A. | Immersion | W.D. (mm) | Type | Type | Thread |
| 1 | U Plan S Apochromat | 10x | 0.4 | Dry | - | - | M23 | |
| 2 | U Plan S Apochromat | 20x | 0.75 | Dry | - | - | M23 | |
| 3 | U Plan Apochromat | 40x | 1 | Oil | 0.12 | Ph3 | Iris | M23 |
| 4 | Plan Apochromat N | 60x | 1.42 | Oil | - | - | M23 | |
| 5 | U Plan S Apochromat | 100x | 1.4 | Oil | - | - | M23 | |
| 6 | Plan Apochromat N | 2x | 0.08 | Dry | - | - | M27 |
Fluorescent Filter Sets:
| Position | Name | Excitation | Dichroic | Emission | Suitable Dyes |
| 1 | DAPI - 5060C | 377/50 | 409LP | 447/60 | DAPI, Hoechst |
| 2 | GFP-4050A | 466/40 | 495LP | 525/50 | EGFP, Alexa Fluor 488 |
| 3 | mCherry-40LP-A | 562/40 | 593LP | 593LP |
mCherry, Alexa Fluor 568 |
| 4 | CFP-2432C | 438/24 | 458LP | 483/32 | ECFP, mCerulean |
| 5 | YFP-2427B | 500/24 | 520LP | 542/27 | EYFP, mVenus |
| 6 | - | - | - | - | - |
Computer:
Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU
1TB Raid Array
30" LCD Monitor
Software:
NIS Elements F
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
CZI Scientist
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Deborah Barkauskas
Senior Microscopist
+61 7 334 62042
d.barkauskas@imb.uq.edu.au
Dr Mahdie Mollazade
Microscopy Officer
+61 7 334 62042
m.mollazade@uq.edu.au
Mailing address
ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia