Fluoro 1

Upright Olympus BX51

Fluoro 1 Training Manual           Location: Rm 4.022           Bookings

 

 

 

 

 

 

 

Olympus BX51 Upright Microscope Stand with Nikon Digital Sight DS-Fi3 CCD camera

Upright widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.

Features Include:

  • Manual X-Y-Z stage
  • Polarised light
  • Differential Interference Contrast (DIC)
  • Optovar (1x, 1.25x, 1.6x, 2x)

Transmitted Light:  Olympus 100W Halogen Lamp

Reflected Light:  Olympus 100W Mercury White Lightsource

 

 

 

 

 

 

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6

Position 7

Position 8

Universal 0.9 - Open Open Open 10x DIC 20x DIC 40x DIC 60x DIC 100x DIC

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 2x 0.08 Dry - -   M27
2 Plan Apochromat 10x 0.40 Dry - -   M27
3 Plan Apochromat 20x 0.75 Dry - -   M27
4 UApo 40x 1.35 Oil - -   M27
5 Plan Apochromat 60x 1.35 Oil - -   M27
6 Plan Apochromat 100x 1.4 Oil - -   M27

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI - 5060C 377/50 409LP 447/60 DAPI, Hoechst
2 GFP-4050A 466/40 495LP 525/50 EGFP, Alexa Fluor 488
3 mCherry-40LP-A 562/40 593LP 593LP

mCherry, Alexa Fluor 568

4 CFP-2432C 438/24 458LP 483/32 ECFP, mCerulean
5 YFP-2427B 500/24 520LP 542/27 EYFP, mVenus
6 Empty - - - Brightfield

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

30" LCD Monitor

Software: 

NIS Elements F

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia