Institute for Molecular Bioscience Institute for Molecular Bioscience

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Specialty Microscopes

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BD Pathway1&2

Current Status:  Fully Operational

Resource Links:

Room 6.033 - Custom Inverted Microscope Stand with Hamamatsu Orca CCD camera

Inverted widefield microscope with Dual Mercury lightsources, high speed filter wheels and CCD camera. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features Include:

  • Fully motorised X-Y-Z stage
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging

Transmitted Light: 10W LED Lamp

Reflected Light:  Olympus Mercury White Lightsource

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 4x 0.5 Dry 2 PhL ADL M27
2 Plan Apochromat 10x 0.75 Dry 0.5 Ph1   M27
3 Plan Apochromat 20x 0.75 Dry 0.4 Ph2 ADL M27
4 Plan Apochromat 40x 0.95 Dry 0.2 DIC I   M27

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI - 5060C 377/50 409LP 447/60 DAPI, Hoechst
2 CFP - 2432C 438/24 458LP 483/32 CFP, Cerulean
3 GFP-3000B 488/10 505LP 530/60 GFP, A488
4 mCherry LP 560/20 580LP 585LP mCherry, Cy3
5   - - -  
6   - - -  

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

30" LCD Monitor

Software: 

Pathway

Accessories:

Incubation chamber with CO2 and Temperature control

FLIM/FRET

Current Status:  Fully Operational

Resource Links:

Room 2.138 - Olympus IX71 - Inverted Microscope Stand with Intensified CCD camera

Inverted widefield microscope with Mercury and LED lightsources and CCD camera. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features Include:

  • Manual X-Y-Z stage
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging

Transmitted Light: 100W Halogen Lamp

Reflected Light:  Olympus Mercury White Lightsource, Lambert LED Lightsource

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 4x 0.5 Dry 2 PhL ADL M27
2 Plan Apochromat 10x 0.75 Dry 0.5 Ph1   M27
3 Plan Apochromat 20x 0.75 Dry 0.4 Ph2 ADL M27
4 Plan Apochromat 40x 0.95 Dry 0.2 DIC I   M27

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI - 5060C 377/50 409LP 447/60 DAPI, Hoechst
2 CFP - 2432C 438/24 458LP 483/32 CFP, Cerulean
3 GFP-3000B 488/10 505LP 530/60 GFP, A488
4 mCherry LP 560/20 580LP 585LP mCherry, Cy3
5   - - -  
6   - - -  

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

Dual 27" LCD Monitors

Software: 

Lambert Intruments

Accessories:

Incubation chamber with CO2 and Temperature control

OPT

Current Status:  Fully Operational

Resource Links:

​OPT

Room 6.025d - Custom Microscope Stand with Photometrics Coolsnap HQ2 CCD camera

Custom widefield microscope with Whitelight Mercury lightsource and CCD camera. Suited for imaging large fixed samples in 3D.

Features Include:

  • Fully motorised X-Y-Z stage

Transmitted Light: 100W LED Lamp

Reflected Light:  Exfo Xacte 200W Mercury White Lightsource

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 0.18x 0.04 Dry 50      
2 Plan Apochromat 0.5x 0.1 Dry 45      
3 Plan Apochromat 1x 0.2 Dry 40      

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI - 5060C 377/50 409LP 447/60 DAPI, Hoechst
2 CFP - 2432C 438/24 458LP 483/32 CFP, Cerulean
3 GFP-3000B 488/10 505LP 530/60 GFP, A488
4 mCherry LP 560/20 580LP 585LP mCherry, Cy3
5   - - -  
6   - - -  

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

30" LCD Monitor

Software: 

OPTiscan