Dr Anne Lagendijk
Dr Anne Lagendijk
Group Leader

Guardians of leakage: understanding how blood vessels control blood flow

Our vascular system transports approximately 7500 liters of blood each day. Arteries deliver oxygen and nutrients throughout the body after which the venous system returns the deoxygenated blood back to the heart. Architecturally, these blood vessels are extremely heterogeneous.

Endothelial cells make up the inner lining of all blood vessels. These cells can continuously adapt their size, adhesiveness and compliance in order to ensure the right balance between vessel integrity and permeability. Mechanical cues play a major role in the functional adaptation of the endothelium and aberrant physical cues have been implicated in vascular diseases like stroke, vascular malformations, atherosclerosis and hypertension.

The Lagendijk group aims to understand how mechanical changes are regulated in the vasculature, by identifying and understanding the molecules involved and also by measuring the magnitude of forces that are transduced.

Publications

1. Lagendijk AK, Gomez GA, Baek S, Hesselson D, Hughes WE, Paterson S, Conway DE, Belting H-G, Affolter M, Smith KA, Schwartz MA, Yap AS*, Hogan BM*. (2017). Live imaging molecular changes in junctional tension upon VE-cadherin in zebrafish. Nature Communications.

2. De Angelis JE*, Lagendijk AK*, Chen H, Tromp A, Bower NI, Tunny KA, Brooks AJ, Bakkers J, Francois M, Yap AS, Simons C, Wicking C, Hogan BM, Smith KA. (2017). Tmem2 Regulates Embryonic Vegf Signaling by Controlling Hyaluronic Acid Turnover. Developmental Cell.

3. Koltowska K*, Lagendijk AK*, Pichol-Thievend C, Fischer JC, Francois M, Ober EA, Yap AS, Hogan BM. (2015). Vegfc Regulates bi-potential precursor division and Prox1 expression to promote lymphatic identity in zebrafish. Cell Reports.

4. Lagendijk AK, Yap AS, Hogan BM. (2018). Notching a new pathway in vascular flow sensing. Trends in Cell Biology.

5. Grassini DR, Lagendijk AK, De Angelis JE, da Silva J, Jeanes A, Zettler N, Bower NI, Hogan BM, Smith KA. (2018). Nppa and Nppb act redundantly during zebrafish cardiac development to confine AVC marker expression and reduce cardiac volume. Development.

6. Paatero I, Sauteur L, Lee M, Lagendijk AK, Heutschi D, Bieli D, Hogan BM, Affolter M* and Belting H-G*. (2018). Junction-based lamellipodia drive endothelial cell rearrangements in vivo via a VE-cadherin/F-actin based oscillatory ratchet mechanism. Nature Communications.

7. Lagendijk AK, Hogan BM. (2015). VE-cadherin in vascular development: a coordinator of cell signaling and tissue morphogenesis. Current Topics in Developmental Biology. IMB Review Submission, 2018 – Appendix 8 Page | 101

8. Lagendijk AK, Goumans MJ, Burkhard S, Bakkers J (2011). MicroRNA-23 restricts cardiac valve formation by inhibiting Has2 and extracellular hyaluronic acid production. Circulation Research.

9. Kloosterman WP, Lagendijk AK, Ketting RF, Moulton JD, Plasterk RH (2007). Targeted inhibition of miRNA maturation with morpholinos reveals a role for miR-375 in pancreatic islet development. PLoS Biology.

10. Lagendijk AK, Moulton JD, Bakkers J (2012). Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues. Biology Open.