Nikon Live Imager
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Room 6.025c - Nikon Ti-E Inverted Microscope Stand with Hamamatsu Flash 4.0 sCMOS camera
Inverted widefield microscope with Lumencor 7 line LED lightsource, high speed filter wheels and CMOS camera. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.
Features Include:
- Fully motorised X-Y-Z stage
- Piezo Z-drive for rapid Z-stacks
- CO2 and temperature incubation for live cells and tissue
- Tiled imaging
- Multi-position imaging
Transmitted Light: Lumencor LIDA
Reflected Light: Lumencor Spectra LED Lightsource
Condensor:
| Name | N.A. | W.D. mm | Position 1 | Position 2 | Position 3 | Position 4 | Position 5 | Position 6 |
| Universal LWD | 0.45 | 54 | - | - | - | - | - | - |
Objectives:
| Position | Objective | Magnification | N.A. | Immersion | W.D. (mm) | Type | Type | Thread |
| 1 | Plan Apochromat | 4x | 0.20 | Dry | - | - | M27 | |
| 2 | Plan Apochromat λ | 10x | 0.45 | Dry | 4 | DIC Ι | M27 | |
| 3 | Plan Apochromat VC | 20x | 1.10 | Dry | 1 | DIC ΙΙ | M27 | |
| 4 | Plan Apochromat λ | 40x | 0.95 | Dry | 0.25 | DIC ΙΙ | M27 | |
| 5 | Plan Fluor | 40x | 1.30 | Oil | 0.22 | DIC ΙΙ | M27 | |
| 6 | Plan Apochromat λ | 60x | 1.40 | Oil | 0.13 | Ph3 | M27 |
Fluorescent Filters:
| Position | Name | Excitation | Dichroic | Emission | Suitable Dyes |
| 1 | Quad band pass | 390/18 | 410/504/582/669 | 440/521/607/700 | DAPI, eGFP, RFP, Cy5 |
| 2 | Triple band pass | 513/17 | 436/514/604 | 525/30 | GFP, YFP, Cy5 |
| 3 | CFP/YFP | 438/24 | 462/523 | 440/40 | CFP, YFP |
| 4 | GFP/RFP | 485/20 | 505/606 | 479/40 | eGFP, RFP |
| 5 | GFP only | 560/25 | 488LP | 542/27 | eGFP, FITC, Alexa 488 |
LEDs:
|
Designation |
Wavelength |
Power |
BFP Power* |
Suitable Dyes |
|
390 |
390nm |
- |
- |
DAPI, Hoechst |
|
440 |
440nm |
- |
- |
CFP |
|
485 |
485nm |
- |
- |
GFP, A488 |
|
514 |
514nm |
- |
- |
YFP |
|
563 |
563nm |
- |
- |
mCherry, Cy3 |
|
594 |
594nm |
- |
- |
A594 |
|
640 |
640nm |
- |
- |
Cy5, A647 |
*BFP = Back Focal Plane of Objective
Computer:
Dell PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU
3TB Raid Array
30" LCD Monitor
Software:
NIS Elements 4.3
Accessories:
Incubation chamber with CO2 and Temperature control
3 x slide holder
4 x 35 mm dish holder
Custom 3D printed holder options
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
Research Officer, Microscopy
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Deborah Barkauskas
Senior Microscopist
+61 7 334 62042
d.barkauskas@imb.uq.edu.au
Dr Tevin Chau
Microscopy Officer
+61 7 334 62042
c.chau@uq.edu.au
Mailing address
ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia