Nikon Live Imager

Nikon Decon Quick Start Guide           Nikon Filter Chart            Location: Rm 6.025c           Bookings

 

 

 

 

 

 

Room 6.025c - Nikon Ti-E Inverted Microscope Stand with Hamamatsu Flash 4.0 sCMOS camera

Inverted widefield microscope with Lumencor 7 line LED lightsource, high speed filter wheels and CMOS camera. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features Include:

  • Fully motorised X-Y-Z stage
  • Piezo Z-drive for rapid Z-stacks
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging

Transmitted Light:  Lumencor LIDA

Reflected Light:  Lumencor Spectra LED Lightsource

 

 

 

 

 

 

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.45 54 - - - - - -

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 4x 0.20 Dry - -   M27
2 Plan Apochromat  λ 10x 0.45 Dry 4 DIC Ι   M27
3 Plan Apochromat VC 20x 1.10 Dry 1 DIC ΙΙ   M27
4 Plan Apochromat  λ 40x 0.95 Dry 0.25 DIC ΙΙ   M27
5 Plan Fluor 40x 1.30 Oil 0.22 DIC ΙΙ   M27
6 Plan Apochromat λ 60x 1.40 Oil 0.13 Ph3   M27

Fluorescent Filters:

Position Name Excitation Dichroic Emission Suitable Dyes
1 Quad band pass 390/18 410/504/582/669 440/521/607/700 DAPI, eGFP, RFP, Cy5
2 Triple band pass 513/17 436/514/604 525/30 GFP, YFP, Cy5
3 CFP/YFP 438/24 462/523 440/40 CFP, YFP
4 GFP/RFP 485/20 505/606 479/40 eGFP, RFP
5 GFP only 560/25 488LP 542/27 eGFP, FITC, Alexa 488

LEDs:

Designation

Wavelength

Power

BFP Power*

Suitable Dyes

390

390nm

-

-

DAPI, Hoechst

440

440nm

-

-

CFP

485

485nm

-

-

GFP, A488

514

514nm

-

-

YFP

563

563nm

-

-

mCherry, Cy3

594

594nm

-

-

A594

640

640nm

-

-

Cy5, A647

*BFP = Back Focal Plane of Objective

Computer:

Dell PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU

3TB Raid Array

30" LCD Monitor

Software: 

NIS Elements 4.3

Accessories:

Incubation chamber with CO2 and Temperature control

3 x slide holder

4 x 35 mm dish holder

Custom 3D printed holder options

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Ms Tevin Chau
Microscopy Officer
  +61 7 334 62042
  c.chau@uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia