Zeiss Live Imager 2

 

 

 

 

 

 

 

Room 6.025a - Zeiss Axio Observer 7 inverted Microscope Stand with Apotome 2, Prime BSI and Axiocam 705 camera

Inverted widefield microscope with Colibri 7 lightsource and CCD camera. Suited for live fluorescent samples.

Features

  • Motorised X-Y stage
  • 500 um Piezo Z control
  • Apotome2
  • Definite Focus 2

Transmitted Light:  LED illumination

Reflected Light:  Colibri 7 lightsource

 

 

 

 

 

 

 

 

 

 

 

Condensor:

Name

N.A.

W.D. mm

Position 1

Position 2

Position 3

Position 4

Position 5

Position 6

Motorised Universal

0.9

 

 

DIC I

DIC II

DIC III

 

 

Objectives:

Position

Objective

Magnification

N.A.

Immersion

W.D. (mm)

Type

Type

Thread

1

EC Plan NeoFluar

5x

0.17

Dry

18.5

Ph1

 

M27

2

Plan Apochromat

10x

0.45

Dry

2.1

 

 

M27

3

EC-Plan Neofluar

20x

0.50

Dry

2

Ph2

 

M27

4

EC-Plan Neofluar

40x

0.75

Dry

0.71

Ph2

 

M27

5

Plan Apochromat

40x

1.30

Oil

0.21

DIC

 

M27

6

Plan Apochromat

63x

1.40

Oil

0.19

DIC

 

M27

 

Fluorescent Filter Sets:

Position

Name

Excitation

Dichroic

Emission

Suitable Dyes

1

DAPI 5060C

377

409

447/60

DAPI, Hoechst

2

FITC 44

475

500

530/50

EGFP, Alexa Fluor 488

3

Cy3 63 He

572

590

629/62

Cy3

4

Cy5 50

640

660

690/50

Cy5, A647

5

Penta Band

Penta Band

Penta Band

Penta Band

DAPI,FITC,TRITC,Cy5, Cy7

6

Polariser

-

-

-

Brightfield

 

Computer:

HP Z8 G4 PC - 3GHz Xeon Processor -192GB RAM - NVIDIA Quadro P4000 GPU

1TB Raid Array

30" LCD Monitor

 

Software: 

Zeiss Zen Blue 3 with Guided Acquisition and Experiment Designer

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
CZI Imaging Scientist
  +61 7 334 62042
  n.condon@imb.uq.edu.au

 

Dr Mahdie Mollazade
Microscopy Officer
  +61 7 334 62042
  m.mollazade@uq.edu.au

 

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia