Zeiss Live Imager 2
Room 6.025a - Zeiss Axio Observer 7 inverted Microscope Stand with Apotome 2, Orca Flash 4.0 and Axiocam 705 camera
Inverted widefield microscope with Colibri 7 lightsource and CCD camera. Suited for live fluorescent samples.
Features
Motorised X-Y-Z stage, Apotome2
Transmitted Light: LED illumination
Reflected Light: Colibri 7 lightsource
Condensor:
|
Name |
N.A. |
W.D. mm |
Position 1 |
Position 2 |
Position 3 |
Position 4 |
Position 5 |
Position 6 |
|
Motorised Universal |
0.9 |
|
|
DIC I |
DIC II |
DIC III |
|
|
Objectives:
|
Position |
Objective |
Magnification |
N.A. |
Immersion |
W.D. (mm) |
Type |
Type |
Thread |
|
1 |
EC Plan NeoFluar |
5x |
0.16 |
Dry |
18.5 |
DIC |
|
M27 |
|
2 |
Plan Apochromat |
10x |
0.30 |
Dry |
2 |
DIC |
|
M27 |
|
3 |
Plan Apochromat |
20x |
0.80 |
Dry |
0.55 |
DIC |
|
M27 |
|
4 |
Plan Neofluar |
40x |
1.30 |
Oil |
0.21 |
DIC |
|
M27 |
|
5 |
Plan Apochromat |
63x |
1.40 |
Oil |
0.14 |
DIC |
|
M27 |
|
6 |
Plan Apochromat |
100x |
1.40 |
Oil |
0.17 |
DIC |
|
M27 |
Fluorescent Filter Sets:
|
Position |
Name |
Excitation |
Dichroic |
Emission |
Suitable Dyes |
|
1 |
DAPI 5060C |
377 |
409 |
447/60 |
DAPI, Hoechst |
|
2 |
FITC 44 |
475 |
500 |
530/50 |
EGFP, Alexa Fluor 488 |
|
3 |
Cy3 63 He |
572 |
590 |
629/62 |
Cy3 |
|
4 |
Cy5 50 |
640 |
660 |
690/50 |
Cy5, A647 |
|
5 |
Penta Band |
Penta Band |
Penta Band |
Penta Band |
DAPI,FITC,TRITC,Cy5, Cy7 |
|
6 |
Polariser |
- |
- |
- |
Brightfield |
Computer:
HP Z6 G4 PC - 3GHz Xeon Processor -192GB RAM - NVIDIA Quadro P4000 GPU
1TB Raid Array
30" LCD Monitor
Software:
Zeiss Zen Blue 3 with Tiling and EDF modules
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
Research Officer, Microscopy
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Deborah Barkauskas
Senior Microscopist
+61 7 334 62042
d.barkauskas@imb.uq.edu.au
Mailing address
ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia