Specialty

TBD

OPT Training Manual      Location: Rm 6.025d      Bookings      Contact

 

 

 

 

 

 

Current Status:  Fully Operational

Room 6.033 - Custom Microscope Stand with Photometrics Coolsnap HQ2 CCD camera

Custom widefield microscope with Whitelight Mercury lightsource and CCD camera. Suited for imaging large fixed samples in 3D.

Features

  • Fully motorised X-Y-Z stage

 

Transmitted Light100W LED Lamp

Reflected Light:  Exfo Xacte 200W Mercury White Lightsource

 

 

 

 

 

 

Objectives

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 0.18x 0.04 Dry 50      
2 Plan Apochromat 0.5x 0.1 Dry 45      
3 Plan Apochromat 1x 0.2 Dry 40      

 

Fluorescent Filter Sets

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI - 5060C 377/50 409LP 447/60 DAPI, Hoechst
2 CFP - 2432C 438/24 458LP 483/32 CFP, Cerulean
3 GFP-3000B 488/10 505LP 530/60 GFP, A488
4 mCherry LP 560/20 580LP 585LP mCherry, Cy3
5   - - -  
6   - - -  

 

Computer

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

30" LCD Monitor

 

Software

OPTiscan

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
CZI Scientist
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Dr Mahdie Mollazade
Microscopy Officer
  +61 7 334 62042
  m.mollazade@uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia