Fluoro 2

Upright Zeiss AxioImager

Fluoro 2 Training Manual            Location: Rm 3.021           Bookings

 

 

 

 

 

 

Room 3.021 - Zeiss AxioImage M2.m Microscope Stand with Apotome2 and Axiocam 506 monochrome and colour cameras

Inverted widefield microscope with LED lightsource, and monochrome and CCD camera. Suited for imaging fixed and live fluorescent samples.

Features Include:

Motorised X-Y-Z stage, Apotome2

Transmitted Light:  LED illumination

Reflected Light:  Zeiss Collibri 7

 

 

 

 

 

 

Condensor:

NameN.A.W.D. mmPosition 1Position 2Position 3Position 4Position 5Position 6
Motorised Universal0.9  DIC IDIC IIDIC III  

Objectives:

PositionObjectiveMagnificationN.A.ImmersionW.D. (mm)TypeTypeThread
1EC Plan NeoFluar4x0.16Dry18.5DIC M27
2Plan Apochromat10x0.45Dry2.1DIC M27
3Plan Apochromat20x0.80Dry0.55DIC M27
4Plan Neofluar40x0.95Dry0.25DIC M27
5Plan Apochromat63x1.40Oil0.19DIC M27
6Neofluar100x1.40Oil0.20DIC M23

Fluorescent Filter Sets:

PositionNameExcitationDichroicEmissionSuitable Dyes
1     
2DAPI 5060C377/50409447/60DAPI, Hoechst
3Zeiss #44 HE475/40500530/50EGFP, Alexa Fluor 488
3Zeiss #63 He572/25590629/62Cy3, mCherry, Texas Red
4Zeiss #50 He640/30660690/50Cy5, A647
5Zeiss #112 He385/30, 469/38, 555/30, 631/33, 735/40405, 493, 575, 654, 761425/30, 514/31, 592/25, 681/45, 785/38DAPI,FITC,TRITC,Cy5, Cy7
6Polariser----

Computer:

HP Z820 PC - 3GHz Xeon Processor -64GB RAM - 1GB ATI Firepro V5800 GPU

1TB Raid Array

30" LCD Monitor

Software: 

Zeiss Zen Blue 3 with Tiling and EDF modules

Accessories:

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Dr Tevin Chau
Microscopy Officer
  +61 7 334 62042
  c.chau@uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia