Fluoro 2
Upright Zeiss AxioImager
Room 3.021 - Zeiss AxioImage M2.m Microscope Stand with Apotome2 and Axiocam 506 camera
Inverted widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.
Features Include:
Motorised X-Y-Z stage, Apotome2
Transmitted Light: LED illumination
Reflected Light: HXP 120c White Lightsource
Condensor:
| Name | N.A. | W.D. mm | Position 1 | Position 2 | Position 3 | Position 4 | Position 5 | Position 6 |
| Motorised Universal | 0.9 | DIC I | DIC II | DIC III |
Objectives:
| Position | Objective | Magnification | N.A. | Immersion | W.D. (mm) | Type | Type | Thread |
| 1 | EC Plan NeoFluar | 5x | 0.16 | Dry | 18.5 | DIC | M27 | |
| 2 | Plan Apochromat | 10x | 0.30 | Dry | 2 | DIC | M27 | |
| 3 | Plan Apochromat | 20x | 0.80 | Dry | 0.55 | DIC | M27 | |
| 4 | Plan Neofluar | 40x | 1.30 | Oil | 0.21 | DIC | M27 | |
| 5 | Plan Apochromat | 63x | 1.40 | Oil | 0.14 | DIC | M27 | |
| 6 | Plan Apochromat | 100x | 1.40 | Oil | 0.17 | DIC | M27 |
Fluorescent Filter Sets:
| Position | Name | Excitation | Dichroic | Emission | Suitable Dyes |
| 1 | DAPI 5060C | 377 | 409 | 447/60 | DAPI, Hoechst |
| 2 | FITC 44 | 475 | 500 | 530/50 | EGFP, Alexa Fluor 488 |
| 3 | Cy3 63 He | 572 | 590 | 629/62 | Cy3 |
| 4 | Cy5 50 | 640 | 660 | 690/50 | Cy5, A647 |
| 5 | Penta Band | Penta Band | Penta Band | Penta Band | DAPI,FITC,TRITC,Cy5, Cy7 |
| 6 | Polariser | - | - | - | Brightfield |
Computer:
HP Z820 PC - 3GHz Xeon Processor -64GB RAM - 1GB ATI Firepro V5800 GPU
1TB Raid Array
30" LCD Monitor
Software:
Zeiss Zen Blue 3 with Tiling and EDF modules
Accessories:
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
Research Officer, Microscopy
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Deborah Barkauskas
Senior Microscopist
+61 7 334 62042
d.barkauskas@imb.uq.edu.au
Mailing address
ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia