Fluoro 2

Upright Zeiss AxioImager

Fluoro 2 Training Manual            Location: Rm 3.021           Bookings

 

 

 

 

 

 

Room 3.021 - Zeiss AxioImage M2.m Microscope Stand with Apotome2 and Axiocam 506 monochrome and colour cameras

Inverted widefield microscope with LED lightsource, and monochrome and CCD camera. Suited for imaging fixed and live fluorescent samples.

Features Include:

Motorised X-Y-Z stage, Apotome2

Transmitted Light:  LED illumination

Reflected Light:  Zeiss Collibri 7

 

 

 

 

 

 

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Motorised Universal 0.9     DIC I DIC II DIC III    

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 EC Plan NeoFluar 4x 0.16 Dry 18.5 DIC   M27
2 Plan Apochromat 10x 0.45 Dry 2.1 DIC   M27
3 Plan Apochromat 20x 0.80 Dry 0.55 DIC   M27
4 Plan Neofluar 40x 0.75 Dry 0.71 DIC   M23
5 Plan Apochromat 63x 1.40 Oil 0.19 DIC   M27
6 Neofluar 100x 1.40 Oil 0.20 DIC   M23

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1          
2 DAPI 5060C 377/50 409 447/60 DAPI, Hoechst
3 Zeiss #44 HE 475/40 500 530/50 EGFP, Alexa Fluor 488
3 Zeiss #63 He 572/25 590 629/62 Cy3, mCherry, Texas Red
4 Zeiss #50 He 640/30 660 690/50 Cy5, A647
5 Zeiss #112 He 385/30, 469/38, 555/30, 631/33, 735/40 405, 493, 575, 654, 761 425/30, 514/31, 592/25, 681/45, 785/38 DAPI,FITC,TRITC,Cy5, Cy7
6 Polariser - - - -

Computer:

HP Z820 PC - 3GHz Xeon Processor -64GB RAM - 1GB ATI Firepro V5800 GPU

1TB Raid Array

30" LCD Monitor

Software: 

Zeiss Zen Blue 3 with Tiling and EDF modules

Accessories:

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Ms Tevin Chau
Microscopy Officer
  +61 7 334 62042
  c.chau@uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia