Fluoro 2

Upright Olympus BX51

Fluoro 2 Training Manual            Zeiss Filters            Location: Rm 3.021           Bookings

 

 

 

 

 

 

Room 3.021 - Zeiss AxioImage M2.m Microscope Stand with Apotome2 and Axiocam 506 camera

Inverted widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.

Features Include:

Motorised X-Y-Z stage, Apotome2

Transmitted Light:  LED illumination

Reflected Light:  HXP 120c White Lightsource

 

 

 

 

 

 

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Motorised Universal 0.9     DIC I DIC II DIC III    

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 EC Plan NeoFluar 5x 0.16 Dry 18.5 DIC   M27
2 Plan Apochromat 10x 0.30 Dry 2 DIC   M27
3 Plan Apochromat 20x 0.80 Dry 0.55 DIC   M27
4 Plan Neofluar 40x 1.30 Oil 0.21 DIC   M27
5 Plan Apochromat 63x 1.40 Oil 0.14 DIC   M27
6 Plan Apochromat 100x 1.40 Oil 0.17 DIC   M27

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI 5060C 377 409 447/60 DAPI, Hoechst
2 FITC 44 475 500 530/50 EGFP, Alexa Fluor 488
3 Cy3 63 He 572 590 629/62 Cy3
4 Cy5 50 640 660 690/50 Cy5, A647
5 Penta Band Penta Band Penta Band Penta Band DAPI,FITC,TRITC,Cy5, Cy7
6 Polariser - - - Brightfield

Computer:

HP Z820 PC - 3GHz Xeon Processor -64GB RAM - 1GB ATI Firepro V5800 GPU

1TB Raid Array

30" LCD Monitor

Software: 

Zeiss Zen Blue 3 with Tiling and EDF modules

Accessories:

Contacts

Dr James Springfield
Microscopy Facility Manager​
  +61 7 334 62390
  j.springfield@imb.uq.edu.au


Dr Nicholas Condon
Research Officer, Microscopy​
  +61 7 334 62042
  n.condon@imb.uq.edu.au

Dr Deborah Barkauskas
Senior Microscopist​
  +61 7 334 62042
  d.barkauskas@imb.uq.edu.au

Mailing address

ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia