Deltavision
Room 2.136 - Olympus IX80 Inverted Microscope Stand with Photometrics Coolsnap HQ2 camera
Inverted widefield microscope with Lumencor 7 line LED lightsource, high speed filter wheels and CCD camera. Suited for imaging fixed samples on slides, and samples with low fluorescence.
Features Include:
- Fully motorised X-Y-Z stage
- High Speed Z-drive for rapid Z-stacks
- CO2 and temperature incubation for live cells and tissue
- Tiled imaging
- Multi-position imaging
Transmitted Light: 100W LED Lamp
Reflected Light: Lumencor Spectra LED Lightsource
Condensor:
| Name | N.A. | W.D. mm | Position 1 | Position 2 | Position 3 | Position 4 | Position 5 | Position 6 |
| Universal LWD | 0.45 | 54 | Open | PhL | Ph1 | Ph2 | DIC I | DIC II |
Objectives:
| Position | Objective | Magnification | N.A. | Immersion | W.D. (mm) | Type | Type | Thread |
| 1 | U-Apochromat | 40x | 1.35 | Oil | - | DIC | M27 | |
| 2 | Plan Apochromat | 60x | 1.35 | Oil | - | DIC | M27 | |
| 3 | UPLS-Apochromat | 100x | 1.4 | Oil | - | DIC | M27 | |
| 4 | - | - | - | - | - | - | ||
| 5 | - | - | - | - | - | - | ||
| 6 | - | - | - | - | - | - |
Fluorescent Filter Sets:
| Position | Name | Excitation | Dichroic | Emission | Suitable Dyes |
| 1 | DAPI | 360/40 | Quad Band | 457/40 | DAPI, Hoechst |
| 2 | FITC | 490/20 | Quad Band | 526/38 | EGFP, Alexa Fluor 488 |
| 3 | TRITC | 555/28 | Quad Band | 617/63 | mCherry, Alexa Fluor 555 |
| 4 | Cy5 | 640/20 | Quad Band | 685/40 | Cy5, Alexa Fluor 647 |
LEDs:
| LED |
Wavelength |
Laser Power |
BFP Power* |
Suitable Dyes |
|
390 |
390nm |
20mW |
4.5mW |
DAPI / Hoechst |
|
440 |
440nm |
5mW |
1mW |
CFP |
|
485 |
485nm |
20mW |
8mW |
GFP / A488 |
|
514 |
514nm |
3mW |
1mW |
YFP |
|
563 |
563nm |
10mW |
5mW |
mCherry / Cy3 |
|
594 |
594nm |
5mW |
1mW |
A594 |
|
640 |
640nm |
5mW |
2mW |
Cy5 / A647 |
*BFP = Back Focal Plane of Objective
Computer:
Dell PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU
1TB Raid Array
30" LCD Monitor
Software:
Softworx
Contacts
Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au
Dr Nicholas Condon
CZI Scientist
+61 7 334 62042
n.condon@imb.uq.edu.au
Dr Deborah Barkauskas
Senior Microscopist
+61 7 334 62042
d.barkauskas@imb.uq.edu.au
Dr Mahdie Mollazade
Microscopy Officer
+61 7 334 62042
m.mollazade@uq.edu.au
Mailing address
ACRF Cancer Biology Imaging Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia