Training Microscopes (L)

Inverted Olympus IX80

FL3 Training Guide Location: Rm 6.033 Bookings

Olympus IX80 Inverted Microscope Stand with Nikon DS-FI3 sCMOS camera

Inverted widefield microscope with Halogen lightsource and sCMOS camera. Suited for imaging fixed fluorescent samples.

Features Include:

Manual X-Y-Z stage
Brightfield & DIC
Fluorescence
Colour Camera

Transmitted Light: Olympus 100W Halogen Lamp

Reflected Light: XCite 120Q Halogen White Lightsource

Condensor:

Name	N.A.	W.D. mm	Position 1	Position 2	Position 3	Position 4	Position 5	Position 6
Universal LWD	0.45	54	Open	PhL	Ph1	Ph2	DIC I	DIC II

Objectives:

Position	Objective	Magnification	N.A.	Immersion	W.D. (mm)	Type	Type	Thread
1	U Plan Fluorite	4x	0.13	Dry	17			M23
2	U Plan Apochromat	10x	0.4	Dry	3.1			M23
3	U Plan S Apochromat	20x	0.75	Dry	0.65			M23
4	U Plan FLN	40x	0.75	Dry	0.51			M23
5	U Plan S Apochromat	63x	1.35	Oil	0.17			M23
6	U Plan Apochromat	100x	1.35	Oil	0.10	DIC III		M23

Fluorescent Filter Sets:

Position	Name	Excitation	Dichroic	Emission	Suitable Dyes
1	DAPI - 5060C	377/50	409LP	447/60	DAPI, Hoechst
2	CFP - 2432C	438/24	458LP	483/32	CFP, Cerulean
3	GFP-3000B	488/10	505LP	530/60	GFP, A488
4	YFP-2427B	500/24	520	542/27	YFP
5	mCherry-40LP	560/20	580LP	585LP	mCherry, Cy3
6	-	-	-	-	-

Computer:

Dell PC - 2.0GHz i5 Processor - 16GB RAM

1TB Raid Array

47" TV Monitor

Software:

Windows 10 Enterprise LTSC

NIS Elements F

Contacts

Dr James Springfield
Microscopy Facility Manager
+61 7 334 62390
j.springfield@imb.uq.edu.au

Dr Nicholas Condon
CZI Imaging Scientist
+61 7 334 62042
n.condon@imb.uq.edu.au

Dr Mahdie Mollazade
Microscopy Officer
+61 7 334 62042
m.mollazade@uq.edu.au

Mailing address

Advanced Microscopy Facility
Institute for Molecular Bioscience
Level 6N, 306 Carmody Road,
Building 80
University of Queensland
4072, St Lucia,
Queensland, Australia

Bookings

IMB internal Microscope and Image Analysis Bookings

UQ staff

External