Joseph Chen*, Christian M. Nefzger*#, Fernando J. Rossello, Yu B.Y. Sun, Sue Mei Lim, Xiaodong Liu, Suzan de Boer Anja S. Knaupp, Jinhua Li, Kathryn C. Davidson, Jose M. Polo#, Tiziano Barberi#. Fine tuning of canonical Wnt stimulation enhances differentiation of pluripotent stem cells independent of β-catenin-mediated TCF signaling. STEM CELLS. 2018 Jun;36(6):822-833. doi: 10.1002/stem.2794. Epub 2018 Feb 14.

 

Pflueger C, Tan D, Swain T, Nguyen T, Pflueger J, Nefzger CM, Polo JM, Ford E, Lister R. A modular dCas9-SunTag DNMT3A epigenome editing system overcomes pervasive off-target activity of direct fusion dCas9-DNMT3A constructs. GENOME RESEARCH. 2018 Aug;28(8):1193-1206. doi: 10.1101/gr.233049.117. Epub 2018 Jun 15.

 

Christian M. Nefzger*, Fernando J. Rossello*, Joseph Chen, Xiaodong Liu, Anja S. Knaupp, Jaber Firas, Jacob M. Paynter, Jahnvi Pflueger, Sam Buckberry, Sue Mei Lim, Brenda Williams, Sara Alaei, Keshav Faye-Chauhan, Enrico Petreto, Susan K. Nilsson, Ryan Lister, Mirana Ramialison, David R. Powel, Owen J.L. Rackham# and Jose M. Polo#. Cell type of origin dictates the route to pluripotency. CELL REPORTS. 2017 Dec 5;21(10):2649-2660. DOI: 10.1016/j.celrep.2017.11.029.

 

Anja S. Knaupp, Sam Buckberry, Jahnvi Pflueger, Sue Mei Lim, Ethan Ford, Michael R. Larcombe, Fernando J. Rossello, Alex de Mendoza, Sara Alaei, Jaber Firas, Melissa L. Holmes, Christian M. Nefzger, Ryan Lister# and Jose M. Polo#. Transient and permanent reconfiguration of chromatin and transcription factor occupancy drive reprogramming. CELL STEM CELL. 2017 Dec 7;21(6):834-845.e6. DOI: 10.1016/j.stem.2017.11.007.

 

Liu X*, Nefzger CM*, Rossello FJ, Chen J, Knaupp AS, Firas J, Ford E, Pflueger J, Paynter JM, Chy HS, O’Brien CM, Huang C, Mishra K, Hodgson-Garms M, Jansz N, Williams SM, Blewitt ME, Nilsson SK, Schittenhelm RB, Laslett AL, Lister R, Polo JM#. Comprehensive characterization of distinct states of human naïve pluripotency generated by reprogramming. NATURE METHODS, 2017 Nov;14(11):1055-1062. DOI: 10.1038/nmeth.4436.

 

Nefzger CM, Polo JM#. DEAD-Box RNA Binding Protein: Not a Black-Box during reprogramming. CELL STEM CELL. 2017 Apr 6;20(4):419-420. DOI: 10.1016/j.stem.2017.03.006.Alaei S, Knaupp AS, Lim SM, Chen J, Holmes ML, Änkö ML, Nefzger CM# and Polo JM#. An improved reprogrammable mouse model harboring the reverse tetracycline-controlled transcriptional transactivator 3. STEM CELL RESEARCH. 2016 Jul;17(1):49-53. DOI: 10.1016/j.scr.2016.05.008.

 

Nefzger CM*, Jarde T*, Horvay K, Knaupp AS, Rosello FJ, Chen J, Powel DR, Abud HE#, Polo JM#. A versatile strategy for isolating a highly enriched population of intestinal stem cells. STEM CELL REPORTS. 2016 Mar 8;6(3):321-9. DOI: 10.1016/j.stemcr.2016.01.014.

 

Owen J.L. Rackham*, Jaber Firas*, Hai Fang, Matt E. Oates, Melissa Holmes, Anja Knaupp, the fantom consortium, Harukazu Suzuki, Christian M Nefzger, Carsten O. Daub, Jay W. Shin,Enrico Petretto, Alistair R.R. Forrest, Yoshihide Hayashizaki, Jose M. Polo#, Julian Gough#. Mogrify: An Atlas for Direct Reprogramming Between Human Cell Types. NATURE GENETICS. 2016. 2016 Mar;48(3):331-5. DOI: 10.1038/ng.3487.

 

Firas J*, Liu X*, Nefzger CM# and Polo JM#. GM-CSF and MEF-conditioned media support feeder-free reprogramming of mouse granulocytes to iPS cells. DIFFERENTIATION. 2014 Jun;87(5):193-9. DOI: 10.1016/j.diff.2014.05.003.

 

Polo JM*, Anderssen E*, Walsh RM, Schwarz BA, Nefzger CM, Lim SM, Borkent M, Apostolou E, Alaei S, Cloutier J, Bar-Nur O, Cheloufi S, Stadtfeld M, Figueroa ME, Robinton D, Natesan S, Melnick A, Zhu J, Ramaswamy S#, Hochedlinger K#. A molecular roadmap of reprogramming somatic cells into iPS cells. CELL. 2012 Dec 21;151(7):1617-32. DOI: 10.1016/j.cell.2012.11.039.

 

Nefzger CM*, Su CT*, Fabb SR, Hartley BJ, Beh SJ, Zeng WR, Haynes JM#, Pouton CW#. Lmx1a allows context-specific isolation of progenitors of GABAergic or dopaminergic neurons during neural differentiation of embryonic stem cells. STEM CELLS. 2012 Jul;30(7):1349-61. DOI: 10.1002/stem.1105.