BrightField/DarkField (BF/DF)

Current Status:  Fully Operational


Resource Links:


Room 4.022 - Olympus BX51 Upright Microscope Stand with Olympus DP70 CCD camera

Upright widefield microscope with Halogen lightsource and CCD camera. Suited for imaging fixed samples on slides.

Features Include:

  • Manual X-Y-Z stage

Transmitted Light:  Olympus 100W Halogen Lamp

Condensor:

Name N.A. W.D. mm
Brightfield 0.90 -

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1

PlanN

2x

0.06

Dry

-

-

 

M27

2

UPlanApo

4x

0.16

Dry

-

-

 

M27

3

PlanN

10x

0.25

Dry

-

-

 

M27

4

UplanSApo

20x

0.75

Dry

-

-

 

M27

5

UPlanSApo

40x

0.90

Dry

-

-

 

M27

6

-

-

-

-

-

-

 

M27

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

30" LCD Monitor

Software: 

DP Manager

 

Fluoro 1

Current Status:  Fully Operational


Resource Links:


Room 4.022 - Olympus BX51 Upright Microscope Stand with Olympus DP70 CCD camera

Upright widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.

Features Include:

  • Manual X-Y-Z stage
  • Polarised light
  • Differential Interference Contrast (DIC)
  • Optovar (1x, 1.25x, 1.6x, 2x)

Transmitted Light:  Olympus 100W Halogen Lamp

Reflected Light:  Olympus 100W Mercury White Lightsource

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6

Position 7

Position 8

Universal 0.9 - Open Open Open 10x DIC 20x DIC 40x DIC 60x DIC 100x DIC

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 2x 0.08 Dry - -   M27
2 Plan Apochromat 10x 0.40 Dry - -   M27
3 Plan Apochromat 20x 0.75 Dry - -   M27
4 UApo 40x 1.35 Oil - -   M27
5 Plan Apochromat 60x 1.35 Oil - -   M27
6 Plan Apochromat 100x 1.4 Oil - -   M27

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI - 5060C 377/50 409LP 447/60 DAPI, Hoechst
2 GFP-4050A 466/40 495LP 525/50 EGFP, Alexa Fluor 488
3 mCherry-40LP-A 562/40 593LP 593LP

mCherry, Alexa Fluor 568

4 CFP-2432C 438/24 458LP 483/32 ECFP, mCerulean
5 YFP-2427B 500/24 520LP 542/27 EYFP, mVenus
6 Empty - - - Brightfield

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

30" LCD Monitor

Software: 

DP Manager, DP Controller

Fluoro 3 - Inverted Olympus IX70

Current Status:  Fully Operational


Resource Links:


Room 2.138 - Olympus IX70 Inverted Microscope Stand with Nikon DS-Fi1 CCD camera

Inverted widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.

Features Include:

  • Manual X-Y-Z stage

Transmitted Light:  Olympus 100W Halogen Lamp

Reflected Light:  XCite 120Q Halogen White Lightsource

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.45 54 Open PhL Ph1 Ph2 DIC I DIC II

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Apochromat 4x 0.5 Dry 2 PhL ADL M27
2 Plan Apochromat 10x 0.75 Dry 0.5 Ph1   M27
3 Plan Apochromat 20x 0.75 Dry 0.4 Ph2 ADL M27
4 Plan Apochromat 40x 0.95 Dry 0.2 DIC I   M27
5 Plan Apochromat 63x 1.4 Oil 0.17 DIC II   M27
6 Plan Apochromat 100x 1.4 Oil 0.13 DIC III   M27

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI - 5060C 377/50 409LP 447/60 DAPI, Hoechst
2 CFP - 2432C 438/24 458LP 483/32 CFP, Cerulean
3 GFP-3000B 488/10 505LP 530/60 GFP, A488
4 mCherry LP 560/20 580LP 585LP mCherry, Cy3
5   - - -  
6   - - -  

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

30" LCD Monitor

Software: 

NIS Elements

Fluoro 4 - Inverted Nikon Ti-U

Current Status:  Fully Operational


Resource Links:


Room 6.025a - Nikon Ti-U Inverted Microscope Stand with Nikon DS-Fi1 and Qi1 CCD cameras

Inverted widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.

Features Include:

  • Manual X-Y-Z stage

Transmitted Light:  Nikon 100W Halogen Lamp

Reflected Light:  Nikon 100W Mercury White Lightsource

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.45 54 Open PhL Ph1 Ph2 DIC I DIC II

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 Plan Fluor 4x 0.13 Dry - PhL DL M27
2 Plan Fluor 10x 0.30 Dry - Ph1 DL M27
3 Plan Fluor ELWD 20x 0.45 Dry - Ph1 ADL M27
4 Plan Fluor ELWD 40x 0.60 Dry - Ph2 ADL M27
5 Plan Fluor 100x 1.30 Oil - Ph3 DDL M27
6 - - - - - - - M27

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI - 5060C 377/50 409LP 447/60 DAPI, Hoechst
2 CFP - 2432C 438/24 458LP 483/32 ECFP, Cerulean
3 GFP-3000B 488/10 505LP 530/60 EGFP, Alexa Fluor 488
4 YFP-2427B 500/24 520LP 542/27 EYFP, mVenus
5 mCherry-40LP-A 562/40 593LP 593LP mCherry Alexa Fluor 568
6 Empty - - - Brightfield

Computer:

Dell PC - 3GHz Xeon Processor - 8GB RAM - 256GB nVidia Quadro GPU

1TB Raid Array

30" LCD Monitor

Software: 

NIS Elements-BR, NIS Elements F

Accessories:

Motorised Z-Focus

Fluoro 5 - Upright Zeiss AxioImager

Current Status:  Fully Operational


Resource Links:


Room 3.011 - Zeiss AxioImage M2.m Microscope Stand with Apotome2 and Axiocam 506 camera

Inverted widefield microscope with Mercury lightsource and CCD camera. Suited for imaging fixed and live fluorescent samples.

Features Include:

Motorised X-Y-Z stage, Apotome2

Transmitted Light:  LED illumination

Reflected Light:  HXP 120c White Lightsource

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Motorised Universal 0.9     DIC I DIC II DIC III    

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 EC Plan NeoFluar 5x 0.16 Dry 18.5 DIC   M27
2 Plan Apochromat 10x 0.30 Dry 2 DIC   M27
3 Plan Apochromat 20x 0.80 Dry 0.55 DIC   M27
4 Plan Neofluar 40x 1.30 Oil 0.21 DIC   M27
5 Plan Apochromat 63x 1.40 Oil 0.14 DIC   M27
6 Plan Apochromat 100x 1.40 Oil 0.17 DIC   M27

 

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI 49 365 395 445/50 DAPI, Hoechst
2 FITC 44 475/40 500 530/50 EGFP, Alexa Fluor 488
3 Cy3 43 545/25 570 605/70 Cy3
4 Texas Red 45 560/40 585 630/75 Tx Red, mCherry, A568
5 Cy5 50 640/30 660 690/50 Cy5, A647
6 Empty - - - Brightfield

 

Computer:

HP Z820 PC - 3GHz Xeon Processor -64GB RAM - 1GB ATI Firepro V5800 GPU

1TB Raid Array

30" LCD Monitor

 

Software: 

Zeiss Zen 2 with Tiling and EDF modules

Accessories: