Institute for Molecular Bioscience Institute for Molecular Bioscience
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Mr Alun Jones

Manager, Mass Spectrometry Facility & Professional Manager
Institute for Molecular Bioscience
+61 7 334 62996

Alun has more than 28 years of experience in biological mass spectrometry in both academia and industry in the UK, Canada and USA. During this time, he has developed HPLC-mass spectrometry interfaces, methods of analysis for natural products, drug and pesticide analysis, peptide and protein analysis.

Publications

Book Chapter (1)
Journal Articles (76)
Conference Papers (5)

Book Chapter

Multiple reaction monitoring for the accurate quantification of amino acids: using hydroxyproline to estimate collagen content

Journal Articles

Proteomic and functional variation within black snake venoms (Elapidae: Pseudechis)
Differential protein expression marks the transition from infection with opisthorchis viverrini to cholangiocarcinoma
Venom profiling of a population of the theraphosid spider Phlogius crassipes reveals continuous ontogenetic changes from juveniles through adulthood
Combining sense and nonsense codon reassignment for site-selective protein modification with unnatural amino acids
A comparative proteomic study of drought-tolerant and drought-sensitive soybean seedlings under drought stress
Deep venomics of the Pseudonaja genus reveals inter- and intra-specific variation
Mammalian farnesyltransferase α subunit regulates vacuolar protein sorting-associated protein 4A (Vps4A) – dependent intracellular trafficking through recycling endosomes
Flexibility versus rigidity for orally bioavailable cyclic hexapeptides
Optimised deep-targeted proteotranscriptomic profiling reveals unexplored conus toxin diversity and novel cysteine frameworks
PGTools: a software suite for proteogenomic data analysis and visualization
A defined α-Helix in the bifunctional O-Glycosylated Natriuretic Peptide TcNPa from the venom of Tropidechis carinatus
Urinary biomarkers for detection of early and advanced chronic kidney disease-A pilot study
Extreme venom variation in Middle Eastern vipers: A proteomics comparison of Eristicophis macmahonii, Pseudocerastes fieldi and Pseudocerastes persicus
A HIV-1 Tat mutant protein disrupts HIV-1 Rev function by targeting the DEAD-box RNA helicase DDX1
Clawing through evolution: toxin diversification and convergence in the ancient lineage Chilopoda (Centipedes)
Multifunctional warheads: diversification of the toxin arsenal of centipedes via novel multidomain transcripts
Solution structure, membrane interactions and protein binding partners of the tetraspanin Sm-TSP-2, a vaccine antigen from the human blood fluke Schistosoma mansoni
Vintage venoms: proteomic and pharmacological stability of snake venoms stored for up to eight decades
Single-step protease cleavage elution for identification of protein-protein interactions from GST pull-down and mass spectrometry
Proteomic comparison of Hypnale hypnale (Hump-Nosed Pit-Viper) and Calloselasma rhodostoma (Malayan Pit-Viper) venoms
Venom proteomic characterization and relative antivenom neutralization of two medically important Pakistani elapid snakes (Bungarus sindanus and Naja naja)
Dracula's children: molecular evolution of vampire bat venom
Squeezers and leaf-cutters: differential diversification and degeneration of the venom system in toxicoferan reptiles
Deep venomics reveals the mechanism for expanded peptide diversity in cone snail venom
A proteomics and transcriptomics investigation of the venom from the Barychelid spider Trittame loki (brush-foot trapdoor)
Mass landscapes of seven scorpion species: the first analyses of Australian species with 1,5-DAN matrix
The weak complex between RhoGAP protein ARHGAP22 and signal regulatory protein 14-3-3 Has 1:2 stoichiometry and a single peptide binding mode
Challenges in mass spectrometry-based quantification of bioactive peptides: A case study exploring the neuropeptide Y family
Eukaryotic elongation factor 1 complex subunits are critical HIV-1 reverse transcription cofactors
Macrophage secretory products induce an inflammatory phenotype in hepatocytes
Albumins and their processing machinery are hijacked for cyclic peptides in sunflower
A novel strategy for the targeted analysis of protein and peptide metabolites
Lectin magnetic bead array for biomarker discovery
Clathrin-independent carriers form a high capacity endocytic sorting system at the leading edge of migrating cells
Exposed proteins of the Schistosoma japonicum tegument
The secreted and surface proteomes of the adult stage of the carcinogenic human liver fluke Opisthorchis viverrini
Rapid extraction combined with LC-tandem mass spectrometry (CREM-LC/MS/MS) for the determination of ciguatoxins in ciguateric fish flesh (vol 54, pg 62, 2009)
Nucleophosmin and nucleolin regulate K-Ras plasma membrane interactions and MAPK signal transduction
A Granulin-Like Growth Factor Secreted by the Carcinogenic Liver Fluke, Opisthorchis viverrini, Promotes Proliferation of Host Cells
Remarkable inter- and intra-species complexity of conotoxins revealed by LC/MS
Comparison of the peptidome and insecticidal activity of venom from a taxonomically diverse group of theraphosid spiders
Rapid extraction combined with LC-tandem mass spectrometry (CREM-LC/MS/MS) for the determination of ciguatoxins fish flesh
A sensitive LC/MS/MS assay of 25OH vitamin D₃ and 25OH vitamin D₂ in dried blood spots
Hydroxyproline quantification for the estimation of collagen in tissue using multiple reaction monitoring mass spectrometry
Quantitative analysis of backbone-cyclised peptides in plants
Differential proteomic analysis of bovine conceptus fluid proteins in pregnancies generated by assisted reproductive technologies
Identification of Disulfide-containing chemical cross-links in proteins using MALDI-TOF/TOF-MAss spectrometry
Cloning, expression, purification and characterization of a DsbA-like protein from Wolbachia pipientis
Cyclic tetrapeptides via the ring contraction strategy: Chemical techniques useful for their identification
Proteomic analysis of bovine conceptus fluids during early pregnancy
Characterisation of inosine monophosphate dehydrogenase expression during retinal development: Differences between variants and isoforms
Ni2+-based immobilized metal ion affinity chromatography of lactose operon repressor protein from Escherichia Coli
Expression and crystallization of DsbA from Staphylococcus aureus
Identification of immuno-reactive proteins from a sheep gastrointestinal nematode, Trichostrongylus colubriformis, using two-dimensional electrophoresis and mass spectrometry
Novel neutrophil-derived proteins in bronchoalveolar lavage fluid indicate an exaggerated inflammatory response in pediatric cystic fibrosis patients
Feedback regulation of xylem cytokinin content is conserved in pea and arabidopsis
In situ neutralization in boc-chemistry solid phase peptide synthesis: Rapid, high yield assembly of difficult sequences
Identification of the dominant translation start site in the attB1 sequence of the pET-DEST42 Gateway vector
Adiponectin multimerization is dependent on conserved lysines in the collagenous domain: Evidence for regulation of multimerization by alterations in posttranslational modifications
A recombinant diheme SoxAX cytochrome - Implications for the relationship between EPR signals and modified heme-ligands
Peptide quantification by matrix-assisted laser desorption ionisation time-of-flight mass spectrometry: Investigations of the cyclotide kalata B1 in biological fluids
Formation of mononuclear and chloro-bridged binuclear copper(II) complexes of patellamide D, a naturally occurring cyclic peptide: influence of anion and solvent
Chemical and functional identification and characterization of novel sulfated alpha-conotoxins from the cone snail Conus anemone
Synthesis, structure elucidation, in vitro biological activity, toxicity, and Caco-2 cell permeability of lipophilic analogues of α-conotoxin MII
Determination of transport in the Caco-2 cell assay of compounds varying in lipophilicity using LC-MS: enhanced transport of Leu-enkephalin analogues
Characterisation of multiple Caribbean ciguatoxins and congeners in individual specimens of horse-eye jack (Caranx latus) by high-performance liquid chromatography/mass spectrometry
The development and application of a novel safety-catch linker for BOC-based assembly of libraries of cyclic peptides
Conotoxin TVIIA, a novel peptide from the venom of Conus tulipa - 1. Isolation, characterization and chemical synthesis
Novel omega-conotoxins from Conus catus discriminate among neuronal calcium channel subtypes
HPLC/Tandem Electrospray Mass Spectrometry for the Determination of Sub-ppb Levels of Pacific and Caribbean Ciguatoxins in Crude Extracts of Fish
alpha-conotoxin EpI, a novel sulfated peptide from Conus episcopatus that selectively targets neuronal nicotinic acetylcholine receptors
Cooliatoxin, the first toxin from Coolia monotis (dinophyceae)
Characterisation of individual N-and O-linked glycosylation sites using Edman degradation
Crystal Structure, Electrospray Ionization Mass Spectrometry, Electron Paramagnetic Resonance and Magnetic Susceptibility Study of [Cu2(ascidH2) (1,2-mCO3)(H2O)2].2H2O, the Biscopper (II) Complex of Ascidiacyclamide (ascidH4), a Cyclic Peptide Isolated fr
Binding of copper(II) to the cyclic octapeptide patellamide D
Ion-spray tandem mass spectrometry in peptide synthesis: Structural characterization of minor by-products in the synthesis of ACP(65-74)

Conference Papers

The bio-logic of venom complexity
Centipede venoms: Old and unusual
Understanding the chemical diversity of spider venoms using a combined genomic, transcriptomic and proteomic approach
Proteomic systems analysis of the proximal fluid from chronic wounds implies perturbation of oxidative systems and persistent neutrophil influx
Rapid identification of DTSSP crosslinked peptides using LCMALDI Tof-Tof mass spectrometry