Resource Links:


Room 6.025b - Olympus IX80 Inverted Microscope Stand with Photometrics Coolsnap HQ2 camera

Inverted widefield microscope with Lumencor 7 line LED lightsource, high speed filter wheels and CCD camera. Suited for imaging fixed samples on slides, samples with low fluorescence and live imaging.

Features Include:

  • Fully motorised X-Y-Z stage
  • High Speed Z-drive for rapid Z-stacks
  • CO2 and temperature incubation for live cells and tissue
  • Tiled imaging
  • Multi-position imaging

Transmitted Light100W LED Lamp

Reflected Light:  Lumencor Spectra LED Lightsource

Condensor:

Name N.A. W.D. mm Position 1 Position 2 Position 3 Position 4 Position 5 Position 6
Universal LWD 0.45 54 Open PhL Ph1 Ph2 DIC I DIC II

Objectives:

Position Objective Magnification N.A. Immersion W.D. (mm) Type Type Thread
1 U-Apochromat 40x 1.35 Oil - DIC   M27
2 Plan Apochromat 60x 1.35 Oil - DIC   M27
3 UPLS-Apochromat 100x 1.4 Oil - DIC   M27
4 - - - - - -    
5 - - - - - -    
6 - - - - - -    

Fluorescent Filter Sets:

Position Name Excitation Dichroic Emission Suitable Dyes
1 DAPI 360/40 Quad Band 457/40 DAPI, Hoechst
2 FITC 490/20 Quad Band 526/38 EGFP, Alexa Fluor 488
3 TRITC 555/28 Quad Band 617/63 mCherry, Alexa Fluor 555
4 Cy5 640/20 Quad Band 685/40 Cy5, Alexa Fluor 647

LEDs:

Laser

Wavelength

Laser Power

BFP Power*

Suitable Dyes

390

390nm

20mW

4.5mW

DAPI / Hoechst

440

440nm

5mW

1mW

CFP

485

485nm

20mW

8mW

GFP / A488

514

514nm

3mW

1mW

YFP

563

563nm

10mW

5mW

mCherry / Cy3

594

594nm

5mW

1mW

A594

640

640nm

5mW

2mW

Cy5 / A647

*BFP = Back Focal Plane of Objective

Computer:

Dell PC - 3GHz Xeon Processor - 32GB RAM - 1GB nVidia Quadro GPU

1TB Raid Array

30" LCD Monitor

Software: 

Softworx

Accessories:

Incubation chamber with CO2 and Temperature control